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首页> 外文期刊>Cell motility and the cytoskeleton >Myofibrillogenesis in skeletal muscle cells in the presence of taxol.
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Myofibrillogenesis in skeletal muscle cells in the presence of taxol.

机译：紫杉醇存在下骨骼肌细胞的肌原纤维形成。

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We address the controversy of whether mature myofibrils can form in the presence of taxol, a microtubule-stabilizing compound. Previous electron microscopic studies reported the absence of actin filaments and Z-bands in taxol-treated myocytes [Antin et al., 1981: J Cell Biol 90:300-308; Toyoma et al., 1982: Proc Natl Acad Sci USA 79:6556-6560]. Quail skeletal myoblasts were isolated from 10-day-old embryos and grown in the presence or absence of taxol. Taxol inhibited the formation of multinucleated elongated myotubes. Myocytes cultured in the continual presence of taxol progressed from rounded to stellate shapes. Groups of myocytes that were clustered together after the isolation procedure fused in the presence of taxol but did not form elongated myotubes. Actin filaments and actin-binding proteins were detected with several different fluorescent probes in all myofibrils that formed in the presence of taxol. The Z-bands contained both alpha-actinin and titin, and the typical arrays of A-Bands were always associated with actin filaments in the myofibrils. Myofibril formation was followed by fixing cells each day in culture and staining with probes for actin, muscle-specific alpha-actinin, myosin II, nebulin, troponin, tropomyosin, and non-muscle myosin II. Small linear aggregates of alpha-actinin or Z-bodies, premyofibrils, were detected at the edges of the myocytes and in the arms of the taxol-treated cells and were always associated with actin filaments. Non-muscle myosin II was detected at the edges of the taxol-treated cells. Removal of the taxol drug led to the cells assuming a normal compact elongated shape. During the recovery process, additional myofibrils formed at the spreading edges of these elongated and thicker myotubes. Staining of these taxol-recovering cells with specific fluorescent reagents reveals three different classes of actin fibers. These results are consistent with a model of myofibrillogenesis that involves the transition of premyofibrils to mature myofibrils. Cell Motil. Cytoskeleton 58:39-52, 2004.
机译：我们解决了在紫杉醇（一种微管稳定化合物）的存在下是否可以形成成熟的肌原纤维的争论。先前的电子显微镜研究报道了在紫杉醇处理的肌细胞中不存在肌动蛋白丝和Z带[Antin等人，1981：J Cell Biol 90：300-308; Maryland等，1981]。 Toyoma等，1982：Proc Natl Acad Sci USA 79：6556-6560]。从10天大的胚胎中分离出鹌鹑骨骼成肌细胞，并在存在或不存在紫杉醇的情况下使其生长。紫杉酚抑制多核细长肌管的形成。在紫杉醇连续存在下培养的心肌细胞从圆形发展到星状。在紫杉醇的存在下，分离过程之后聚集在一起的成群的心肌细胞融合，但没有形成细长的肌管。在紫杉醇存在下形成的所有肌原纤维中，用几种不同的荧光探针检测到肌动蛋白丝和肌动蛋白结合蛋白。 Z波段同时包含α-肌动蛋白和肌动蛋白，典型的A波段阵列始终与肌原纤维中的肌动蛋白丝相关。形成肌原纤维后，每天固定培养中的细胞，并用肌动蛋白，肌肉特异性α-肌动蛋白，肌球蛋白II，星云蛋白，肌钙蛋白，原肌球蛋白和非肌球蛋白II的探针染色。在肌细胞的边缘和紫杉醇处理的细胞的臂中检测到α-肌动蛋白或Z体的小的线性聚集体，即肌原纤维，并始终与肌动蛋白丝相关。在紫杉醇处理的细胞边缘检测到非肌肉肌球蛋白II。紫杉醇药物的去除导致细胞呈正常紧凑的细长形状。在恢复过程中，额外的肌原纤维在这些细长且较厚的肌管的扩散边缘形成。用特定的荧光试剂对这些回收紫杉醇的细胞进行染色，可以发现三种不同类型的肌动蛋白纤维。这些结果与涉及原肌原纤维向成熟肌原纤维转变的肌原纤维形成模型一致。细胞动力。细胞骨架58：39-52，2004。

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