• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell motility and the cytoskeleton >Microtubules become more dynamic but not shorter during preprophase band formation: A possible 'search-and-capture' mechanism for microtubule translocation.
【24h】

Microtubules become more dynamic but not shorter during preprophase band formation: A possible 'search-and-capture' mechanism for microtubule translocation.

机译：微管变得更动态，但在前期带形成过程中不会变短：一种可能的“搜索和捕获”机制，用于微管移位。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The dynamic behavior of the microtubule cytoskeleton plays a crucial role in cellular organization, but the physical mechanisms underlying microtubule (re)organization in plant cells are poorly understood. We investigated microtubule dynamics in tobacco BY-2 suspension cells during interphase and during the formation of the preprophase band (PPB), the cytoskeletal structure that defines the site of cytokinesis. Here we show that after 2 h of microtubule accumulation in the PPB and concurrent disappearance elsewhere in the cortex, the PPB is completed and starts to breakdown exponentially already 20 min before the onset of prometaphase. During formation of the PPB, the dynamic instability, i.e., the stochastic alternating between growing and shrinking phases, of the cortical microtubules outside the PPB increases significantly, but the microtubules do not become shorter. Based on this, as well as on the cross-linking of microtubules in the PPB and the lack of evidence for motor involvement, we propose a"search-and-capture" mechanism for PPB formation, in which the regulation of dynamic instability causes the cortical microtubules to become more dynamic and possibly longer, while the microtubule cross-linking activity of the developing PPB preferentially stabilizes these "searching" microtubules. Thus, microtubules gradually disappear from the cortex outside the PPB and aggregate to the forming PPB. Cell Motil. Cytoskeleton 57:246-258, 2004.
机译：微管细胞骨架的动态行为在细胞组织中起着至关重要的作用，但对植物细胞中微管（重新）组织的物理机制了解甚少。我们调查了烟草BY-2悬浮细胞在相间和前相前带（PPB）形成过程中的微管动力学，前相带定义了胞质分裂的位点。在这里，我们显示在PPB中2h的微管积聚并在皮层其他地方同时消失后，PPB已完成，并且在前中期开始前20分钟就已经开始以指数方式分解。在PPB形成期间，PPB外部的皮质微管的动态不稳定性，即生长期和收缩期之间的随机交替显着增加，但微管不会变短。基于此，以及PPB中微管的交联和缺乏运动参与的证据，我们提出了PPB形成的“搜索和捕获”机制，其中动态不稳定性的调节导致了PPB的形成。皮层微管变得更加动态，甚至可能更长，而正在发育的PPB的微管交联活性则优先稳定了这些“搜索”的微管。因此，微管从PPB外的皮质逐渐消失，并聚集到形成的PPB中。细胞动力。 Cytoskeleton 57：246-258，2004。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号