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首页> 外文期刊>Cell motility and the cytoskeleton >Morphological irregularities and features of resistance to apoptosis in the dcp-1/pita double mutated egg chambers during Drosophila oogenesis.
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Morphological irregularities and features of resistance to apoptosis in the dcp-1/pita double mutated egg chambers during Drosophila oogenesis.

机译：果蝇卵形成过程中dcp-1 / pita双突变卵室中细胞形态的不规则性和抗凋亡的特征。

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摘要

In the present study, we demonstrate the most novel characteristic morphological features of Drosophila egg chambers lacking both dcp-1 and pita functions in the germline cells. Dcp-1 is an effector caspase and it has been previously shown to play an important role during Drosophila oogenesis [McCall and Steller, 1998 : Science 279 : 230-234; Laundrie et al., 2003 : Genetics 165 : 1881-1888; Peterson et al., 2003 : Dev Biol 260 : 113-123]. The completion of sequencing and annotation of the Drosophila genome has revealed that the dcp-1 gene is nested within an intron of another distinct gene, called pita, a member of the C2H2 zinc finger protein family that regulates transcriptional initiation. The dcp-1(-/-)/pita(-/-) nurse cells exhibit euchromatic nuclei (delay of apoptosis) during the late stages of oogenesis, as revealed by conventional light and electron microscopy. The phalloidin-FITC staining discloses significant defects in actin cytoskeleton arrangement. The actin bundles fail to organize properly and the distribution of actin filaments in the ring canals is changed compared to the wild type. The oocyte and the chorion structures have been also modified. The oocyte nucleus is out of position and the chorion appears to contain irregular foldings, while the respiratory filaments obtain an altered morphology. The dcp-1(-/-)/pita(-/-) egg chambers do not exhibit the rare events of spontaneously induced apoptosis, observed for the wild type flies, during mid-oogenesis. Interestingly, the mutated egg chambers are protected by staurosporine-induced apoptosis in a percentage of 40%, strongly suggesting the essential role of dcp-1 and/or pita during mid-oogenesis.
机译：在本研究中，我们证明了在种系细胞中缺乏dcp-1和pita功能的果蝇卵室的最新颖的特征形态特征。 Dcp-1是一种半胱天冬酶效应物，并且先前已经证明它在果蝇卵子发生过程中起着重要的作用[McCall和Steller，1998：科学279：230-234; McCall和Steller，1998：Science 279：230-234。 Laundrie等，2003：遗传学165：1881-1888;和Peterson等，2003：Dev Biol 260：113-123]。果蝇基因组测序和注释的完成表明，dcp-1基因嵌套在另一个独特基因的内含子中，该基因称为pita，它是调节转录起始的C2H2锌指蛋白家族的成员。 dcp-1（-/-）/ pita（-/-）保育细胞在卵子发生的晚期阶段表现出正常的细胞核（凋亡延迟），这是常规的光学和电子显微镜所揭示的。鬼笔环肽-FITC染色显示肌动蛋白细胞骨架排列存在明显缺陷。与野生型相比，肌动蛋白束无法正确组织，肌动蛋白丝在环管中的分布发生了变化。卵母细胞和绒毛膜结构也已被修饰。卵母细胞核位置不正确，绒毛膜似乎有不规则的折叠，而呼吸丝的形态发生了改变。 dcp-1（-/-）/ pita（-/-）卵腔在卵中生过程中没有观察到野生型果蝇自发诱导凋亡的罕见事件。有趣的是，突变的卵腔受到星形孢菌素诱导的凋亡的40％的保护，强烈暗示了dcp-1和/或皮塔饼在成卵中期的基本作用。

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