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首页> 外文期刊>Cell motility and the cytoskeleton >Rho family GTPases are activated during HGF-stimulated prostate cancer-cell scattering.
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Rho family GTPases are activated during HGF-stimulated prostate cancer-cell scattering.

机译：Rho家族GTPases在HGF刺激的前列腺癌细胞扩散过程中被激活。

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An important process in embryogenesis and cancer-cell metastasis is the conversion of epithelial cells to a migratory phenotype, a phenomenon known as epithelial-mesenchymal transition (E-MT). To achieve E-MT, cells dissociate from neighbouring cells and adopt a migratory morphology. This transition requires remodelling of their cell shape and substratum adhesions; activities that require extensive reorganisation of the actin cytoskeleton. Hepatocyte growth factor (HGF)-induced scattering of Madin Darby canine kidney (MDCK) cells is a routinely used model of E-MT, in which actin cytoskeletal rearrangement is known to be dependent on Rho family GTPases. We have developed a novel model of HGF-induced E-MT using the human prostate cancer cell line, DU145. This model overcomes the limitation of using a canine cell line and facilitates the study of E-MT in human cancer. We demonstrate for the first time the scattering response of individual DU145 cells to HGF in real time and have characterised changes in actin cytoskeletal organisation and cell adhesions as these cells respond to HGF. HGF-induced scattering of DU145 cells is dependent on the activity of Rho family GTPases, and using this model, we are able to demonstrate for the first time that endogenous Cdc42 is activated downstream of HGF. Furthermore we have also shown that the response of DU145 cells to HGF is dependent on a phosphatidylinositide 3-kinase pathway. Cell Motil. Cytoskeleton 62:180-194, 2005. (c) 2005 Wiley-Liss, Inc.
机译：胚胎发生和癌细胞转移的重要过程是上皮细胞向迁移表型的转化，这种现象称为上皮-间质转化（E-MT）。为了实现E-MT，细胞与邻近细胞解离并采取迁移形态。这种转变需要重塑它们的细胞形状和基质粘附。需要大量重组肌动蛋白细胞骨架的活动。肝细胞生长因子（HGF）诱导的Madin Darby犬肾（MDCK）细胞散射是E-MT的常用模型，其中肌动蛋白的细胞骨架重排依赖于Rho家族GTPases。我们已经开发了使用人类前列腺癌细胞系DU145的HGF诱导的E-MT的新型模型。该模型克服了使用犬细胞系的局限性，并促进了人类癌症中E-MT的研究。我们首次证明了单个DU145细胞对HGF的实时散射反应，并表征了肌动蛋白细胞骨架组织和细胞粘附的变化，因为这些细胞对HGF做出了反应。 HGF诱导的DU145细胞的散射取决于Rho家族GTPases的活性，并且使用此模型，我们能够首次证明内源Cdc42在HGF的下游被激活。此外，我们还表明DU145细胞对HGF的反应取决于磷脂酰肌醇3激酶途径。细胞动力。 Cytoskeleton 62：180-194，2005.（c）2005 Wiley-Liss，Inc.

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