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首页> 外文期刊>Cell motility and the cytoskeleton >Cyclooxygenase and cAMP-dependent protein kinase reorganize the actin cytoskeleton for motility in HeLa cells.
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Cyclooxygenase and cAMP-dependent protein kinase reorganize the actin cytoskeleton for motility in HeLa cells.

机译：环氧合酶和cAMP依赖性蛋白激酶可重组肌动蛋白细胞骨架，促进HeLa细胞的运动。

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摘要

The adhesion of a cell to its surrounding matrix is a key determinant in many aspects of cell behavior. Adhesion consists of distinct stages : attachment, cell spreading, motility, and/or immobilization. Interrelated signaling pathways regulate these stages, and many adhesion-related signals control the architecture of the cytoskeleton. The various cytoskeletal organizations then give rise to the specific stages of adhesion. It has been shown that arachidonic acid acts at a signaling branch point during cell attachment. Arachidonic acid is metabolized via lipoxygenase to activate actin polymerization and cell spreading. It is also metabolized by cyclooxygenase to generate small actin bundles. We have used confocal microscopy and indirect immunofluorescence to investigate the structure of these cyclooxygenase dependent actin bundles in HeLa cells. We have also employed cell migration assays and pharmacological modulation of cyclooxygenase and downstream signals. The results indicate that cyclooxygenase and PKA stimulate the formation of actin bundles that contain myosin II and associate with small focal adhesions. In addition, we demonstrate that this cytoskeletal organization correlates with increased cell motility. Cell Motil. Cytoskeleton 55:265-277, 2003.
机译：细胞与其周围基质的粘附是细胞行为许多方面的关键决定因素。粘附包括不同的阶段：附着，细胞扩散，运动和/或固定。相关的信号传导途径调节这些阶段，许多与粘附有关的信号控制细胞骨架的结构。然后，各种细胞骨架组织引起粘附的特定阶段。已经显示花生四烯酸在细胞附着过程中在信号传导分支点起作用。花生四烯酸通过脂氧合酶代谢以激活肌动蛋白聚合和细胞扩散。它也被环氧合酶代谢产生小的肌动蛋白束。我们已经使用共聚焦显微镜和间接免疫荧光来研究HeLa细胞中这些依赖于环氧合酶的肌动蛋白束的结构。我们还采用了细胞迁移测定以及环氧合酶和下游信号的药理调节。结果表明，环氧合酶和PKA刺激肌动蛋白束的形成，其中肌动蛋白束包含肌球蛋白II，并与小的粘着斑相关。另外，我们证明该细胞骨架组织与增加的细胞运动性相关。细胞动力。 Cytoskeleton 55：265-277，2003。

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