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首页> 外文期刊>Cell motility and the cytoskeleton >Localization of two IQGAPs in cultured cells and early embryos of Xenopus laevis.
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Localization of two IQGAPs in cultured cells and early embryos of Xenopus laevis.

机译：在非洲爪蟾的培养细胞和早期胚胎中两个IQGAP的定位。

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Mammalian IQGAP1 is considered to modulate organization of the actin cytoskeleton under regulation of signaling proteins Cdc42 or Rac and calmodulin [Bashour et al., 1997: J Cell Biol 137:1555-1566; Hart et al., 1996: EMBO J 15:2997-3005] and also to be involved in cadherin-based cell adhesion [Kuroda et al., 1998: Science 281:832-835]. However, its function in the cell has not been clear. In order to clarify the function of IQGAP, we investigated IQGAP in Xenopus laevis cells. We isolated two Xenopus cDNAs encoding homologues of mammalian IQGAP, XIQGAP1, and XIQGAP2, which show high homology with human IQGAP1 and IQGAP2, respectively. Immunofluorescent localization of XIQGAPs in Xenopus tissue cultured cells (XTC cells) and in developing embryos was examined. In XTC cells, XIQGAP1 was colocalized with F-actin at cell-to-cell contact sites, membrane ruffles in lamellipodia, and filopodia. During development of embryos, XIQGAP1 was concentrated in the borders of all embryonic cells. An intense staining for XIQGAP1 was found in regions undergoing active morphogenetic movements, such as the blastopore lip of gastrulae, and the neural plate, the notochord, and the somite of neurulae. These results suggest that XIQGAP1 is involved in both cell-to-cell adhesion and cell migration during Xenopus embryogenesis and in cultured cells. On the other hand, the localization of XIQGAP2 in XTC cells was distinct from that of XIQGAP1 although it was also seen in lamellipodia, filopodia, and borders between cells. In addition to these regions, strong nuclear staining was observed in both XTC cells and embryonic cells. Cell Motil. Cytoskeleton 55:36-50, 2003.
机译：哺乳动物IQGAP1被认为在信号蛋白Cdc42或Rac和钙调蛋白的调节下调节肌动蛋白细胞骨架的组织[Bashour等人，1997：J Cell Biol 137：1555-1566; Resin等人，1997。 Hart等，1996：EMBO J 15：2997-3005]，并且还参与基于钙粘着蛋白的细胞粘附[Kuroda等，1998：Science 281：832-835]。但是，其在细胞中的功能尚不清楚。为了阐明IQGAP的功能，我们研究了非洲爪蟾细胞中的IQGAP。我们分离了两个非洲爪蟾cDNA，它们分别编码哺乳动物IQGAP，XIQGAP1和XIQGAP2的同源物，它们分别与人IQGAP1和IQGAP2具有高度同源性。检查了非洲爪蟾组织培养细胞（XTC细胞）和发育中的胚胎中XIQGAP的免疫荧光定位。在XTC细胞中，XIQGAP1与F-肌动蛋白共定位于细胞与细胞之间的接触部位，lamellipodia中的膜皱纹和丝状伪足。在胚胎发育过程中，XIQGAP1集中在所有胚胎细胞的边界。 XIQGAP1在活动形态发生运动的区域中被强烈染色，例如腹鹤果的胚孔唇，神经板，脊索和神经节突。这些结果表明，XIQGAP1在非洲爪蟾胚胎发生过程中和培养的细胞中均参与细胞间粘附和细胞迁移。另一方面，XIQGAP2在XTC细胞中的定位不同于XIQGAP1，尽管在层状脂蛋白，丝状伪足以及细胞之间的边界中也可以看到。除这些区域外，在XTC细胞和胚胎细胞中均观察到强核染色。细胞动力。细胞骨架55：36-50，2003。

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