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首页> 外文期刊>Langmuir: The ACS Journal of Surfaces and Colloids >Shape Changes and Vesicle Fission of Giant Unilamellar Vesicles of Liquid-Ordered Phase Membrane Induced by Lysophosphatidylcholine
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Shape Changes and Vesicle Fission of Giant Unilamellar Vesicles of Liquid-Ordered Phase Membrane Induced by Lysophosphatidylcholine

机译：溶血磷脂酰胆碱诱导的液相序相膜大单层膜囊泡的形状变化和囊泡裂变

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Liquid-ordered phase(Zo phase)of lipid membranes has properties that are intermediate between those of liquid-crystalline phase and those of gel phase and has attracted much attention in both biological and biophysical aspects.Rafts in the Zo phase in biomembranes play important roles in cell function of mammalian cells such as signal transduction.In this report,we have prepared giant unilamellar vesicles(GUVs)of lipid membranes in the Zo phase and investigated their physical properties using phase-contrast microscopy and fluorescence microscopy.GUVs of dipalmitoyl-phosphatidylcholine(DPPC)/cholesterol membranes and also GUVs of sphingomyelin(SM)/cholesterol membranes in the Zo phase in water were formed at 20-37 deg C successfully,when these membranes contained >30 mol % cholesterol.The diameters of GUVs of DPPC/cholesterol and SM/cholesterol membranes did not change from 50 to 28 deg C,supporting that the membranes of these GUVs were in the Zo phase.To elucidate the interaction of a substance with a long hydrocarbon chain with the Zo phase membrane,we investigated the interaction of low concentrations(less than critical micelle concentration)of lysophosphatidylcholine(lyso-PC)with DPPC/cholesterol GUVs and SM/cholesterol GUVs in the Zo phase.We found that lyso-PC induced several shape changes and vesicle fission of these GUVs above their threshold concentrations in water.The analysis of these shape changes indicates that lyso-PC can be partitioned into the external monolayer in the Zo phase of the GUV from the aqueous solution.Threshold concentrations of lyso-PC in water to induce the shape changes and vesicle fission increased greatly with a decrease in chain length of lyso-PC.Thermodynamic analysis of this result indicates that shape changes and vesicle fission occur at threshold concentrations of lyso-PC in the membrane.These new findings on GUVs of the Zo phase membranes indicate that substances with a long hydrocarbon chain such as lyso-PC can enter into the Zo phase membrane and also the raft in the cell membrane.We have also proposed a mechanism for the lyso-PC-induced vesicle fission of GUVs.
机译：脂质膜的液序相（Zo相）的性质介于液晶相和凝胶相之间，在生物学和生物物理方面都引起了人们的广泛关注.Zo相在生物膜中的筏起着重要的作用。在本研究中，我们制备了Zo相脂质膜的巨大单层囊泡（GUVs），并使用相差显微镜和荧光显微镜研究了它们的物理性质。二棕榈酰磷脂酰胆碱（DPPC）/胆固醇膜以及在水中Zo相中的鞘磷脂（SM）/胆固醇膜的GUV均在20-37摄氏度下成功形成，当这些膜包含> 30 mol％的胆固醇时.DPPC / GUV的直径胆固醇和SM /胆固醇膜在50到28摄氏度之间没有变化，这表明这些GUV的膜处于Zo相。在Zo相膜上具有长烃链的物质，我们研究了低浓度（低于临界胶束浓度）的溶血磷脂酰胆碱（lyso-PC）与Zo相中DPPC /胆固醇GUV和SM /胆固醇GUV的相互作用。 lyso-PC导致这些GUV的数个形状变化和囊泡裂变，使其高于水中的阈值浓度。对这些形状变化的分析表明，lyso-PC可以从水溶液中分为GUV的Zo相中的外部单层。随着溶菌链长度的减少，水中溶菌PC的阈值浓度会引起形状变化和囊泡裂变大大增加，该结果的热力学分析表明，溶菌PC的阈值浓度下会发生形状变化和囊泡裂变。这些有关Zo相膜GUV的新发现表明，具有长烃链的物质（如lyso-PC）可以进入膜中。 Zo相膜以及细胞膜中的筏。我们还提出了溶血PC诱导的GUV囊泡裂变的机制。

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