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首页> 外文期刊>Caries research >Distribution of three cariogenic bacteria in secondary carious lesions around amalgam restorations.
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Distribution of three cariogenic bacteria in secondary carious lesions around amalgam restorations.

机译：三种汞致病菌在汞合金修复体周围的继发性龋齿中的分布。

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摘要

Secondary dental caries remains an unresolved problem in dentistry and little is known of its microbial etiology. The purpose of this study was to compare the distribution of the three most suspected cariogenic groups of bacteria, mutans streptococci. Actinomyces naeslundii genospecies 2 and lactobacilli, in natural secondary caries around amalgam restorations. Extracted teeth with secondary caries were sectioned to obtain three samples that were randomly distributed to three different groups. Each group was immunolabeled with antibodies to either Streptococcus mutans, A. naeslundii genospecies 2 or Lactobacillus casei and subsequently labeled with secondary fluorescent antibodies. All samples were analyzed three-dimensionally using confocal microscopy. The results indicated that the three different bacteria were widely present and could have an important role in the development of secondary caries around amalgam restorations.
机译：继发性龋齿在牙科领域仍未解决，其微生物病因学知之甚少。这项研究的目的是比较三种最可疑的致癌细菌，即变形链球菌，的分布。汞齐修复体周围的天然次生龋中的放线菌基因种2和乳杆菌。将提取的具有继发龋齿的牙齿切成薄片，以获得三个样品，将它们随机分配到三个不同的组。每组均用抗变形链球菌，内氏农杆菌基因种2或干酪乳杆菌的抗体进行免疫标记，然后用第二荧光抗体标记。使用共聚焦显微镜对所有样品进行三维分析。结果表明，这三种不同的细菌广泛存在，并可能在汞合金修复体周围的继发性龋齿的发展中发挥重要作用。

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