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首页> 外文期刊>Caries research >Effect of Apis mellifera propolis from two Brazilian regions on caries development in desalivated rats.
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Effect of Apis mellifera propolis from two Brazilian regions on caries development in desalivated rats.

机译：来自两个巴西地区的蜜蜂蜂胶对脱盐大鼠龋齿发育的影响。

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The purpose of the present study was to evaluate the effect of Apis mellifera propolis collected from two regions of Brazil on caries development in desalivated rats. Ethanolic extracts of propolis (EEP) were prepared from crude propolis samples collected in Minas Gerais state (MG), southeastern Brazil, and Rio Grande do Sul state (RS), southern Brazil. The flavonoid composition of EEP was analyzed by high-performance thin-layer chromatography (HPTLC) and reversed-phase high-performance liquid chromatography (HPLC). For the animal study, 30 specific pathogen-free Wistar rats were infected with Streptococcus sobrinus 6715 and surgically desalivated. The rats were randomly divided into three groups which were treated with 80% ethanol (control), EEP from MG and EEP from RS. The animals were placed in a Konig-Hofer programmed feeder and received 17 meals of diet 2000 daily at hourly intervals. The solutions were applied on the rat molars (25 microl on molars of each quadrant) twice a day, by using graduate syringes. After 3 weeks, the animals were killed by CO(2) asphyxiation. For microbial assessment, the left jaw was removed and sonicated in 154 mM NaCl solution. Dental caries was evaluated according to Larson's modification of Keyes' system. The HPTLC patterns and HPLC profiles demonstrated that both quality and quantity of flavonoid aglycones of EEP from MG were different compared to EEP from RS. In general, it is apparent that EEP from RS contained the highest concentrations of pinocembrin, chrysin, acacetin and galangin. The group of animals treated with EEP from RS showed the lowest smooth-surface and sulcal caries scores as well as less caries severity in smooth-surface and sulcal lesions, and these data were statistically different when compared with the control group. The group treated with EEP from MG only demonstrated a significant difference in the severity of sulcal lesions when compared to the control group. The percentage of S. sobrinus was lower in the groups treated with EEP, but did not differ statistically from the control group. The results showed that the cariostatic effect of propolis depends on its composition, and consequently the region of collection of propolis samples.
机译：本研究的目的是评估从巴西两个地区收集的蜜蜂蜜蜂对脱盐大鼠龋齿发育的影响。蜂胶的乙醇提取物（EEP）是从巴西东南部的米纳斯吉拉斯州（MG）和巴西南部的南里奥格兰德州（RS）采集的粗制蜂胶样品中制备的。通过高效薄层色谱（HPTLC）和反相高效液相色谱（HPLC）分析EEP的类黄酮成分。在动物研究中，将30只无病原体的Wistar大鼠感染了Sobrinus sobrinus 6715链球菌，并进行了手术除盐处理。将大鼠随机分为三组，分别用80％乙醇（对照组），MG的EEP和RS的EEP处理。将动物放置在Konig-Hofer程序的喂食器中，每天以每小时的间隔接受2000顿的17顿饮食。通过使用刻度注射器，每天两次将溶液涂在大鼠的臼齿上（每个象限的臼齿为25微升）。 3周后，动物被CO（2）窒息杀死。为了进行微生物评估，将左颌骨切除并在154 mM NaCl溶液中超声处理。根据Larson对Keyes系统的修改来评估龋齿。 HPTLC模式和HPLC图谱表明，与RS的EEP相比，MG的EEP的类黄酮苷元的质量和数量均不同。通常，很明显，来自RS的EEP含有最高浓度的松胚球蛋白，菊花，乙酰水杨素和高良姜精。用RS的EEP处理的动物组在光滑表面和沟内病变中表现出最低的光滑表面和沟龋得分，并且龋齿严重程度较低，并且与对照组相比，这些数据在统计学上存在差异。与对照组相比，用MG的EEP治疗的组仅表现出沟病变严重程度的显着差异。在用EEP治疗的组中，S。sobrinus的百分比较低，但与对照组无统计学差异。结果表明，蜂胶的抑癌作用取决于蜂胶的成分，因此取决于蜂胶样品的采集区域。

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