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THE SUBCELLULAR LOCALIZATION OF NEWLY SYNTHESIZED CYTOCHROME B(5)

机译:新合成的细胞色素B的亚细胞定位(5)

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The fate of newly synthesized cytochrome b(5) was studied in rat hepatocytes. Using an antibody specific for microsomal cytochrome b(5), we found newly synthesized microsomal cytochrome b(5) in both mitochondria and a mitochondria associated membrane fraction as well as in microsomes. Newly synthesized cytochrome b(5) was quickly removed from the site of synthesis on free ribosomes and inserted into membranes at random. No migration of newly synthesized cytochrome b(5) between cellular compartments was observed and therefore the assembly of the apoprotein with the heme moiety is apparently not taking place in any particular cellular compartment.
机译:在大鼠肝细胞中研究了新合成的细胞色素b(5)的命运。使用特异性针对微粒体细胞色素b(5)的抗体,我们在线粒体和线粒体相关的膜部分以及微粒体中发现了新合成的微粒体细胞色素b(5)。新合成的细胞色素b(5)从游离核糖体上的合成位点快速去除,并随机插入膜中。没有观察到细胞室之间新合成的细胞色素b(5)的迁移,因此载脂蛋白与血红素部分的组装显然不在任何特定的细胞室中发生。

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