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首页> 外文期刊>Cell biology international. >Transfer of transformed chloroplasts from Nicotiana tabacum to the Lycium barbarum plants.
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Transfer of transformed chloroplasts from Nicotiana tabacum to the Lycium barbarum plants.

机译：将转化的叶绿体从烟草中转移到枸杞植物中。

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摘要

Plastid transformation is an attractive technology for obtaining crop plants with new useful characteristics and for fundamental researches of plastid functioning and nuclear-plastid interaction. The aim of our experiments was to obtain plants with Lycium barbarum nucleus and transformed Nicotiana tabacum plastids. Plastome of previously engineered transplastomic tobacco plants contains reporter uidA gene and selective aadA gene that confers resistance to antibiotics spectinomycin and streptomycin. Asymmetric somatic hybridization was performed for transferring transformed tobacco plastids from transplastomic tobacco plants into recipient L. barbarum wild type plants. Hybrid L. barbarum plants containing transformed tobacco plastome with active aadA and uidA genes were obtained as a result of the experiments. The work shows the possibility of obtaining transplastomic plants by transferring the transformed plastids to remote species by using somatic hybridization technology. The developed technique is especially effective for obtaining transplastomic plants that have low regeneration and transformation ability.
机译：质体转化是一种吸引人的技术，可用于获得具有新的有用特性的农作物以及质体功能和核质体相互作用的基础研究。我们的实验目的是获得具有枸杞核和转化烟草质体的植物。先前经过工程改造的转基因烟草植物的质体包含报告基因uidA基因和选择性aadA基因，可赋予对壮观霉素和链霉素抗生素的抗性。进行不对称体细胞杂交以将转化的烟草质体从转质体烟草植物转移到受体L.barbarum野生型植物中。作为实验的结果，获得了含有转化的烟草质体组和活性aadA和uidA基因的杂交L.barbarum植物。这项工作显示了通过使用体细胞杂交技术将转化的质体转移到偏远物种而获得转基因植物的可能性。所开发的技术对于获得具有低再生和转化能力的转基因植物特别有效。

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