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首页> 外文期刊>Cell biology international. >Effects of insulin-like growth factor-1 on okadaic acid-induced apoptosis in SH-SY5Y cells.
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Effects of insulin-like growth factor-1 on okadaic acid-induced apoptosis in SH-SY5Y cells.

机译：胰岛素样生长因子-1对冈田酸诱导的SH-SY5Y细胞凋亡的影响。

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摘要

The effects of insulin-like growth factor-1 (IGF-1) on the cytotoxicity and apoptosis induced by okadaic acid (OA) in SH-SY5Y cells were investigated. Cell viability was measured using the MTT (3-(4,5-dimethylthiazolyl-2)-2,-5-diphenyltetrazolium bromide) assay. Early and late apoptosisecrosis were analyzed by flow cytometry using Annexin V and propidium iodide (PI) double-staining. Caspase-3 activation was detected by Western blot analysis. Preincubation with IGF-1 for 24 h prevented cytotoxicity induced by 40 nM OA given for 24 h, and the MTT value significantly increased. Incubation with 20 nM OA for 24 h caused a marked increase in the percentage of early apoptotic and late apoptoticecrotic cells, which was not dependent on the activation of caspase-3. OA-induced apoptosis was significantly decreased by pretreatment with 10 ng/ml of IGF-1 for 24 h. The results supported the hypothesis that IGF-1 may be useful in the treatment of Alzheimer's disease.
机译：研究了胰岛素样生长因子-1（IGF-1）对冈田酸（OA）诱导的SH-SY5Y细胞的细胞毒性和凋亡的影响。使用MTT（3-（4,5-二甲基噻唑基-2）-2，-5-二苯基四氮唑溴化物）测定法测量细胞活力。使用膜联蛋白V和碘化丙啶（PI）双重染色，通过流式细胞术分析早期和晚期凋亡/坏死。通过蛋白质印迹分析检测到Caspase-3的活化。与IGF-1预温育24小时可防止40 nM OA持续24 h诱导的细胞毒性，并且MTT值显着增加。与20 nM OA一起孵育24小时会导致早期凋亡和晚期凋亡/坏死细胞的百分比显着增加，这与caspase-3的激活无关。通过用10 ng / ml IGF-1预处理24小时，OA诱导的细胞凋亡显着降低。该结果支持IGF-1可能在阿尔茨海默氏病治疗中有用的假设。

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