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首页> 外文期刊>Cell biology international. >FMS*Calciumfluor specifically increases mRNA levels and induces signaling via MAPK 42,44 and not FAK in differentiating rat osteoblasts.
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FMS*Calciumfluor specifically increases mRNA levels and induces signaling via MAPK 42,44 and not FAK in differentiating rat osteoblasts.

机译：FMS *氟化钙在分化大鼠成骨细胞中特异性增加mRNA水平并通过MAPK 42,44而非FAK诱导信号转导。

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摘要

The homeopathic compound of resonance FMS*Calciumfluor (FMS*) reportedly promotes osteogenic differentiation of rat pre-osteoblasts in vitro. Here, we show that the continuous exposure of differentiating rat osteogenic cells (ROB) to FMS* modulates the level of expression of mRNAs for 7 of the 8 osteogenic markers tested. Alkaline phosphatase (AP), osteocalcin (OC), metalloproteinases (MMP-2 and -14), procollagenase C (BMP-1), biglycan (BG) and integrin 1 are expressed at higher levels in FMS*-treated osteoblasts than in control cultures. MMP-2 and -14 mRNA are not down-modulated at mineralization. Also, the pattern of expression induced by FMS* for some of these genes (BMP-1, BG and integrin 1) is changed, but collagen type I (Coll I) mRNA levels are not affected by treatment with FMS*. This suggests that FMS* modulates mRNA levels and that this is not generalized, but gene(s) specific. We also report that exposure to FMS* rapidly and transiently induces activation of mitogen-activated protein kinases(MAPKs) 42,44 in populations of early osteoblasts, but not in pre-osteoblasts, with a cell differentiation stage-dependent and pertussis toxin (PTX)-sensitive response. Subsequent to FMS* MAPK signaling activation, an increase in AP and MMP-14 mRNA is detected, which is also inhibited by PTX, suggesting that FMS* activation of MAPK signaling could be an early event required for the induction of these genes. Exposure to FMS* does not cause changes in the activity of p125 (FAK)-mediated signaling.
机译：据报道，共振FMS *氟化钙（FMS *）的顺势疗法化合物可在体外促进大鼠成骨细胞的成骨分化。在这里，我们显示了分化大鼠成骨细胞（ROB）持续暴露于FMS *可以调节8种成骨标记物中7种的mRNA表达水平。 FMS *处理的成骨细胞中碱性磷酸酶（AP），骨钙蛋白（OC），金属蛋白酶（MMP-2和-14），胶原蛋白原C（BMP-1），双链聚糖（BG）和整联蛋白1的表达水平高于对照组文化。 MMP-2和-14 mRNA在矿化过程中未下调。同样，由FMS *诱导的某些基因（BMP-1，BG和整联蛋白1）的表达模式也发生了变化，但I型胶原（Coll I）mRNA水平不受FMS *处理的影响。这表明FMS *调节mRNA的水平，这不是一般性的，而是基因特异性的。我们还报告说，暴露于FMS *会迅速而短暂地诱导早期成骨细胞群体中的促分裂原活化蛋白激酶（MAPKs）42,44活化，而在成骨前细胞中则没有，具有细胞分化阶段依赖性和百日咳毒素（PTX） ）敏感的响应。在FMS * MAPK信号激活之后，检测到AP和MMP-14 mRNA的增加，这也被PTX抑制，这表明MMS信号的FMS *激活可能是诱导这些基因的早期事件。暴露于FMS *不会引起p125（FAK）介导的信号传导活性的改变。

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