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首页> 外文期刊>Cell biology international. >A microprobe analysis of inorganic elements in Halobacterium salinarum.
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A microprobe analysis of inorganic elements in Halobacterium salinarum.

机译：盐沼中无机元素的微探针分析。

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摘要

Halobacterium salinarum were grown on peptone agar containing 4.28 M NaCl, 0.036 M K and other salts. Stationary phase organisms were lifted onto carbon planchets, freeze-dried, carbon coated and examined in a scanning electron microscope equipped with an X-ray spectrometer. Intracellular element concentrations (mol/kg H(2)O) were determined using a bulk analysis program with appropriate standards. The cell K concentration was 110 times that of the medium. For Na this value was 0.3 and for Cl, 1.1. When Rb was present in the medium, its intracellular concentration was 77 times higher than the external value. The cation minus anion value suggests a high fixed negative charge, 0.72 equivalents. Intracellular apparent dielectric constants were calculated using cellular EMFs derived from the literature, and sodium concentration. The determined values ranged from 22-28 (vs 80 for normal water) suggesting phases of structured cell water. Ionic distributions in these extremophiles are treated according to theclassical principles elucidated by Willard Gibbs and represents a heterogeneous system in thermodynamic equilibrium with the hypersaline environment. Factors to be considered are: (1) composition of Halobacterium and its immobile negative charge; (2) the physicochemical properties of the individual ions (charge, ionic radius, hydration energy, standard chemical potential); (3) the dielectric constant of the dispersion medium (water); and (4) the binding of ions, particularly potassium.
机译：食盐盐杆菌在含有4.28 M NaCl，0.036 M K和其他盐的蛋白a琼脂上生长。将固定相生物举到碳片上，冷冻干燥，涂碳并在配备有X射线光谱仪的扫描电子显微镜中检查。使用大量分析程序和适当的标准确定细胞内元素的浓度（mol / kg H（2）O）。细胞K浓度是培养基的110倍。对于Na，该值为0.3，对于Cl，该值为1.1。当培养基中存在Rb时，其细胞内浓度比外部值高77倍。阳离子负离子值表示固定负电荷较高，为0.72当量。使用源自文献的细胞EMF和钠浓度计算细胞内表观介电常数。所确定的值范围为22-28（相对于普通水为80），表明结构化细胞水的相。根据Willard Gibbs阐明的经典原理处理这些极端微生物中的离子分布，并代表与高盐环境处于热力学平衡的异质系统。需要考虑的因素有：（1）盐杆菌的组成及其不可移动的负电荷; （2）各个离子的物理化学性质（电荷，离子半径，水合能，标准化学势）; （3）分散介质（水）的介电常数; （4）离子，特别是钾的结合。

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