• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >Rat cortex and hippocampus-derived soluble factors for the induction of adipose-derived mesenchymal stem cells into neuron-like cells
【24h】

Rat cortex and hippocampus-derived soluble factors for the induction of adipose-derived mesenchymal stem cells into neuron-like cells

机译：大鼠皮质和海马来源的可溶性因子可诱导脂肪来源的间充质干细胞转化为神经元样细胞

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To simulate brain microenvironment, adipose-derived mesenchymal stem cells (AMSC) were induced to differentiate to neuronal-like cells in rat cortex and hippocampus medium (CoxpHip). First, isolated AMSC were characterized by flow cytometer and the capacity of adipogenesis and osteogenesis. After induction in rat cortex and hippocampus conditioned medium, the cell morphological change was examined and neural marker proteins (b-III-Tubulin, NSE, Nissl body) expression was detected by immunofluorescence staining. A variety of synaptic marker proteins, including GAP43, SHANK2, SHANK3 and Bassoon body, were detected. ELISA was used to measure brain derived neurotrophic factor (BDNF) and nerve growth factor (NGF) secretion at different time-points. AMSCs positively expressed CD13, CD44 and CD90 and could differentiate into osteoblasts or adipocytes. After induction in CoxpHip medium for 14 days, cells had a typical neuronal perikaryal appearance, which was suggestive of neuronal differentiation. After 14 days of CoxpHip treatment, the percentage of cells expressing βIII-Tubulin, NSE and Nissl was 53.9±0.8%, 51.3±1.7% and 16.4±2.1%, respectively. Expression of GAP43, SHANK2, SHANK3 and Bassoon body was detected, indicating synapse formation after treatment in CoxpHip medium. Differentiated AMSCs secreted neurotrophic factors NGF and BDNF. Thus rat cortex and hippocampus-derived soluble factors can induce AMSCs to a neuronal-like phenotype, suggesting that AMSCs have a dual role in supplementing newborn neurons and secreting neurotrophic factors, and therefore could be help as a potential treatment for nervous system diseases.
机译：为了模拟脑微环境，在大鼠皮层和海马培养基（CoxpHip）中诱导脂肪来源的间充质干细胞（AMSC）分化为神经元样细胞。首先，通过流式细胞仪以及成脂和成骨的能力对分离的AMSC进行表征。在大鼠皮层和海马条件培养基中诱导后，检查细胞形态变化，并通过免疫荧光染色检测神经标记蛋白（b-III-微管蛋白，NSE，Niss1体）的表达。检测到多种突触标记蛋白，包括GAP43，SHANK2，SHANK3和Bassoon体。 ELISA用于在不同时间点测量脑源性神经营养因子（BDNF）和神经生长因子（NGF）的分泌。 AMSC阳性表达CD13，CD44和CD90，并可能分化为成骨细胞或脂肪细胞。在CoxpHip培养基中诱导14天后，细胞具有典型的神经元周核外观，提示神经元分化。 CoxpHip处理14天后，表达βIII-微管蛋白，NSE和Nissl的细胞百分比分别为53.9±0.8％，51.3±1.7％和16.4±2.1％。检测到GAP43，SHANK2，SHANK3和巴松管体的表达，表明在CoxpHip培养基中处理后突触形成。分化的AMSC分泌神经营养因子NGF和BDNF。因此，大鼠皮层和海马来源的可溶性因子可以诱导AMSC成为神经元样表型，表明AMSC在补充新生神经元和分泌神经营养因子方面具有双重作用，因此可以作为神经系统疾病的潜在治疗方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号