...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell and Tissue Research >Identification and functional response of interstitial Cajal-like cells from rat mesenteric artery.
【24h】

Identification and functional response of interstitial Cajal-like cells from rat mesenteric artery.

机译：大鼠肠系膜动脉间质Cajal样细胞的鉴定和功能反应。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cells with irregular shapes, numerous long thin filaments, and morphological similarities to the gastrointestinal interstitial cells of Cajal (ICCs) have been observed in the wall of some blood vessels. These ICC-like cells (ICC-LCs) do not correspond to the other cell types present in the arterial wall: smooth muscle cells (SMCs), endothelial cells, fibroblasts, inflammatory cells, or pericytes. However, no clear physiological role has as yet been determined for ICC-LCs in the vascular wall. The aim of this study has been to identify and characterize the functional response of ICC-LCs in rat mesenteric arteries. We have observed ICC-LCs and identified them morphologically and histologically in three different environments: isolated artery, freshly dispersed cells, and primary-cultured cells from the arterial wall. Like ICCs but unlike SMCs, ICC-LCs are positively stained by methylene blue. Cells morphologically resembling methylene-blue-positive cells are also positive for the ICC and ICC-LC markers alpha-smooth muscle actin and desmin. Furthermore, the higher expression of vimentin in ICC-LCs compared with SMCs allows a clear discrimination between these two cell types. At the functional level, the differences observed in the variations of cytosolic free calcium concentration of freshly dispersed SMCs and ICC-LCs in response to a panel of vasoactive molecules show that ICC-LCs, unlike SMCs, do not respond to exogenous ATP and [Arginine](8)-vasopressin.
机译：在某些血管壁上已观察到具有不规则形状的细胞，许多长而细的细丝以及与Cajal胃肠间质细胞（ICC）的形态相似性的细胞。这些ICC样细胞（ICC-LC）与动脉壁中存在的其他细胞类型不对应：平滑肌细胞（SMC），内皮细胞，成纤维细胞，炎性细胞或周细胞。但是，尚未确定血管壁中ICC-LC的明确生理作用。这项研究的目的是确定和表征ICC-LCs在大鼠肠系膜动脉中的功能反应。我们已经观察到了ICC-LC，并在三种不同的环境中从形态和组织学上鉴定了它们：分离的动脉，新鲜分散的细胞和来自动脉壁的原代培养细胞。像ICC一样，但与SMC不同，ICC-LC会被亚甲蓝染色。形态类似于亚甲蓝阳性细胞的细胞也对ICC和ICC-LC标记α-平滑肌肌动蛋白和结蛋白呈阳性。此外，与SMC相比，波形蛋白在ICC-LC中的表达更高，从而可以清楚地区分这两种细胞类型。在功能水平上，响应于一组血管活性分子，新鲜分散的SMC和ICC-LC的胞浆游离钙浓度变化中观察到的差异表明，与SMC不同，ICC-LC不响应外源ATP和[精氨酸]（8）-加压素。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号