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首页> 外文期刊>Cell and Tissue Research >Cell proliferation and cytoarchitectural remodeling during spinal cord reconnection in the fresh-water turtle Trachemys dorbignyi.
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Cell proliferation and cytoarchitectural remodeling during spinal cord reconnection in the fresh-water turtle Trachemys dorbignyi.

机译：在淡水龟Trachemys dorbignyi脊髓重新连接期间的细胞增殖和细胞结构重构。

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In fresh-water turtles, the bridge connecting the proximal and caudal stumps of transected spinal cords consists of regenerating axons running through a glial cellular matrix. To understand the process leading to the generation of the scaffold bridging the lesion, we analyzed the mitotic activity triggered by spinal injury in animals maintained alive for 20-30 days after spinal cord transection. Flow cytometry and bromodeoxyuridine (BrdU)-labeling experiments revealed a significant increment of cycling cells around the lesion epicenter. BrdU-tagged cells maintained a close association with regenerating axons. Most dividing cells expressed the brain lipid-binding protein (BLBP). Cells with BrdU-positive nuclei expressed glial fibrillary acidic protein. As spinal cord regeneration involves dynamic cell rearrangements, we explored the ultra-structure of the bridge and found cells with the aspect of immature oligodendrocytes forming an embryonic-like microenvironment. These cells supported and ensheathed regenerating axons that were recognized by immunocytological and electron-microscopical procedures. Since functional recovery depends on proper impulse transmission, we examined the anatomical axon-glia relationships near the lesion epicenter. Computer-assisted three-dimensional models revealed helical axon-glial junctions in which the intercellular space appeared to be reduced (5-7 nm). Serial-sectioning analysis revealed that fibril-containing processes provided myelinating axon sheaths. Thus, disruption of the ependymal layer elicits mitotic activity predominantly in radial glia expressing BLBP on the lateral aspects of the ependyma. These cycling cells seem to migrate and contribute to the bridge providing the main support and sheaths for regenerating axons.
机译：在淡水龟中，连接横切脊髓的近端和尾端残肢的桥由穿过神经胶质细胞基质的再生轴突组成。为了解导致桥接病变的支架的过程，我们分析了在脊髓横断后存活20-30天的动物中，脊髓损伤触发的有丝分裂活性。流式细胞仪和溴脱氧尿苷（BrdU）标记实验表明，病变中心附近的循环细胞显着增加。标记BrdU的细胞与再生轴突保持紧密联系。大多数分裂细胞表达脑脂质结合蛋白（BLBP）。具有BrdU阳性细胞核的细胞表达神经胶质原纤维酸性蛋白。由于脊髓再生涉及动态细胞重排，因此我们探索了桥的超微结构，并发现了具有未成熟少突胶质细胞的细胞，形成了类似胚胎的微环境。这些细胞支持并包裹着被免疫细胞学和电子显微镜程序识别的再生轴突。由于功能恢复取决于适当的脉冲传输，因此我们检查了病变震中附近的解剖轴突-胶质细胞关系。计算机辅助三维模型揭示了螺旋轴突-神经胶质交界处，其中细胞间空间似乎减少了（5-7 nm）。连续切片分析显示，包含纤维的过程提供了髓鞘轴突鞘。因此，对室管膜层的破坏主要在室管膜侧面的表达BLBP的radial状神经胶质细胞中引起有丝分裂活性。这些循环细胞似乎迁移并有助于桥，从而为轴突再生提供主要支撑和鞘。

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