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首页> 外文期刊>Cell motility and the cytoskeleton >Description and characterization of a chamber for viewing and quantifying cancer cell chemotaxis.
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Description and characterization of a chamber for viewing and quantifying cancer cell chemotaxis.

机译：用于查看和量化癌细胞趋化性的腔室的描述和特征。

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Direct observations of cancer cell invasion underscore the importance of chemotaxis in invasion and metastasis. Yet, there is to date, no established method for real-time imaging of cancer chemotaxis towards factors clinically correlated with metastasis. A chamber has been designed and tested, called the Soon chamber, which allows the direct observation and quantification of cancer cell chemotaxis. The premise for the design of the Soon chamber is the incorporation of a dam, which creates a steep gradient while retaining stability associated with a pressure-driven system. The design is based on the characteristics of cancer cell motility such as relatively low speeds, and slower motility responses to stimuli compared to classical amoeboid cells like neutrophils and Dictyostelium. We tested MTLn3 breast carcinoma cells in the Soon chamber in the presence of an EGF gradient, obtaining hour-long time-lapses of chemotaxis. MTLn3 cells migrated further, more linearly, and at greater speeds within an EGF gradient compared to buffer controls. Computation of the degree of orientation towards the EGF/buffer source showed that MTLn3 cells were significantly more directional toward the EGF gradient compared to buffer controls. Analysis of the time-lapse data obtained during chemotaxis demonstrated that two populations of cancer cells were present. One population exhibited oscillations in directionality occurring at average intervals of 12 min while the second population exhibited sustained high levels of directionality toward the source of EGF. This result suggests that polarized cancer cells can avoid the need for oscillatory path corrections during chemotaxis. Cell Motil. Cytoskeleton 62:27-34, 2005. (c) 2005 Wiley-Liss, Inc.
机译：对癌细胞侵袭的直接观察强调了趋化性在侵袭和转移中的重要性。然而，迄今为止，还没有建立针对癌症与临床上与转移相关的化学趋化性的实时成像的方法。已经设计并测试了一个名为Soon的腔室，该腔室可以直接观察和量化癌细胞的趋化性。 Soon室设计的前提是合并水坝，该坝会产生陡峭的坡度，同时保持与压力驱动系统相关的稳定性。该设计基于癌细胞运动的特征，例如相对较低的速度，以及与嗜中性粒细胞和脉管上皮细胞等经典的类阿波样细胞相比，运动对刺激的反应较慢。我们在存在EGF梯度的情况下在Soon室中测试了MTLn3乳腺癌细胞，获得了趋化性长达一小时的时间。与缓冲液对照相比，MTLn3细胞在EGF梯度内以更快速，更线性和更快的速度迁移。对EGF /缓冲液来源的取向程度的计算表明，与缓冲液对照相比，MTLn3细胞对EGF梯度的定向性明显更高。分析趋化过程中获得的延时数据表明存在两个癌细胞群。一个种群表现出方向性振荡，平均间隔为12分钟，而第二种群表现出持续高水平的朝向EGF来源的定向性。该结果表明极化的癌细胞可以避免在趋化过程中进行振荡路径校正。细胞动力。 Cytoskeleton 62：27-34，2005.（c）2005 Wiley-Liss，Inc.

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