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首页> 外文期刊>Cell motility and the cytoskeleton >The role of RhoA in the regulation of cell morphology and motility.
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The role of RhoA in the regulation of cell morphology and motility.

机译：RhoA在调节细胞形态和运动中的作用。

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摘要

Members of the Rho family of small GTPases are key regulators of the actin cytoskeleton, particularly in relation to the cell shape changes and the adhesion dynamic that drive cell migration. Here, we report the effect of activation or inhibition of the function of RhoA on cell motility and morphology. Both in the presence and the absence of serum, expression of constitutively active RhoA dramatically inhibited L929 fibroblasts' cell motility, and induced a rounding of the cells and a decrease in the number of processes per cell. In contrast, expression of a dominant negative mutant of RhoA had no effect on cell motility or morphology in steady-state conditions with or without serum in the medium. Inhibition of p160ROCK, a kinase effector of RhoA, only partially inhibited cell migration. Conversely, when cells were submitted to a period of serum deprivation followed by addition of serum, inhibition of endogenous RhoA by expression of the dominant negative mutant of RhoA impeded cell motility after serum stimulation. Thus, RhoA activity is required for stimulation of cell locomotion by serum factors. It was also observed that the addition of serum factors to quiescent L929 and NR6wtEGFR fibroblasts resulted in a delayed motility response of several hours compared to the immediately induced morphological changes, indicating the absence of a previously assumed direct correlation between changes in cell motility and cell morphology in response to serum addition. The motility response of L929 and NR6wtEGFR fibroblasts to serum stimulation required protein synthesis.
机译：小型GTP酶的Rho家族成员是肌动蛋白细胞骨架的关键调节剂，特别是在细胞形状变化和驱动细胞迁移的粘附动力学方面。在这里，我们报告激活或抑制RhoA功能对细胞运动性和形态的影响。在有血清和无血清的情况下，组成型活性RhoA的表达均能显着抑制L929成纤维细胞的细胞运动，并诱导细胞变圆并减少每个细胞的进程数。相反，在培养基中有或没有血清的稳态条件下，RhoA显性阴性突变体的表达对细胞运动或形态没有影响。 RhoA激酶效应子p160ROCK的抑制仅部分抑制细胞迁移。相反，当将细胞置于血清剥夺期后再添加血清时，RhoA显性负突变体的表达抑制内源性RhoA会抑制血清刺激后的细胞运动。因此，RhoA活性是通过血清因子刺激细胞运动所需的。还观察到，与立即诱导的形态学变化相比，向静止的L929和NR6wtEGFR成纤维细胞中添加血清因子会导致运动反应延迟数小时，这表明细胞运动性与细胞形态学变化之间没有先前假定的直接相关性响应血清添加。 L929和NR6wtEGFR成纤维细胞对血清刺激的运动反应需要蛋白质合成。

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