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首页> 外文期刊>Cell motility and the cytoskeleton >Differences in morphology of phagosomes and kinetics of acidification and degradation in phagosomes between the pathogenic Entamoeba histolytica and the non-pathogenic Entamoeba dispar.
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Differences in morphology of phagosomes and kinetics of acidification and degradation in phagosomes between the pathogenic Entamoeba histolytica and the non-pathogenic Entamoeba dispar.

机译：致病性变形杆菌和非致病性变形杆菌之间吞噬体的形态差异以及吞噬体的酸化和降解动力学的差异。

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Phagocytosis plays an important role in the pathogenicity of the intestinal protozoan parasite Entamoeba histolytica. We compared the morphology of phagosomes and the kinetics of phagosome maturation using conventional light and electron microscopy and live imaging with video microscopy between the virulent E. histolytica and the closely-related, but non-virulent E. dispar species. Electron micrographs showed that axenically cultivated trophozoites of the two Entamoeba species revealed morphological differences in the number of bacteria contained in a single phagosome and the size of phagosomes. Video microscopy using pH-sensitive fluorescein isothiocynate-conjugated yeasts showed that phagosome acidification occurs within 2 min and persists for >12 h in both species. The acidity of phagosomes significantly differed between two species (4.58 +/- 0.36 or 5.83 +/- 0.38 in E. histolytica or E. dispar, respectively), which correlated well with the differences in the kinetics of degradation of promastigotesof GFP-expressing Leishmania amazonensis. The acidification of phagosomes was significantly inhibited by a myosin inhibitor, whereas it was only marginally inhibited by microtubules or actin inhibitors. A specific inhibitor of vacuolar ATPase, concanamycin A, interrupted both the acidification and degradation in phagosomes in both species, suggesting the ubiquitous role of vacuolar ATPase in the acidification and degradation in Entamoeba. In contrast, inhibitors against microtubules or cysteine proteases (CP) showed distinct effects on degradation in phagosomes between these two species. Although depolymerization of microtubules severely inhibited degradation in phagosomes of E. histolytica, it did not affect degradation in E. dispar. Similarly, the inhibition of CP significantly reduced degradation in phagosomes of E. histolytica, but not in E. dispar. These data suggest the presence of biochemical or functional differences in the involvement of microtubules and proteases in phagosome maturation and degradation between the two species. Cell Motil. Cytoskeleton 62:84-99, 2005. (c) 2005 Wiley-Liss, Inc.
机译：吞噬作用在肠道原生动物寄生虫组织解脂变形虫中起重要作用。我们比较了吞噬体的形态和吞噬体成熟的动力学，使用了常规的光镜和电子显微镜以及带有视频显微镜的实时成像技术，将强毒的溶组织性大肠杆菌和密切相关但无毒的致病性大肠杆菌分离。电子显微照片显示，在两个吞噬动植物中，经过线虫培养的滋养体揭示了单个吞噬体中细菌数量和吞噬体大小的形态差异。使用pH敏感的，异硫氰酸荧光素荧光素结合的酵母菌进行的视频显微镜检查显示，吞噬体酸化发生在2分钟内，并且在两个物种中均持续> 12 h。吞噬体的酸度在两个物种之间显着不同（分别在溶组织性大肠杆菌（E. histolytica）或分离性大肠杆菌（E. dispar）中为4.58 +/- 0.36或5.83 +/- 0.38），这与表达GFP的利什曼原虫前鞭毛体降解动力学的差异密切相关。亚马逊吞噬体的酸化被肌球蛋白抑制剂显着抑制，而微管或肌动蛋白抑制剂仅被微抑制。液泡ATPase的特异性抑制剂伴刀豆球蛋白A中断了两个物种中吞噬体的酸化和降解，表明液泡ATPase在Entamoeba的酸化和降解中普遍存在。相反，针对微管或半胱氨酸蛋白酶（CP）的抑制剂对这两种物种之间吞噬体的降解表现出明显的影响。尽管微管的解聚严重抑制了溶组织性大肠杆菌的吞噬体的降解，但它并没有影响Dispar的降解。类似地，对CP的抑制显着降低了溶组织性大肠杆菌的吞噬体中的降解，但不适用于Dispar。这些数据表明，在两个物种之间，微管和蛋白酶参与吞噬体成熟和降解过程中存在生化或功能差异。细胞动力。 Cytoskeleton 62：84-99，2005.（c）2005 Wiley-Liss，Inc.

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