...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >Molecular Characterization of Gap Region in 28S rRNA Molecules in Brine Shrimp Artemia parthenogenetica and Planarian Dugesia japonica
【24h】

Molecular Characterization of Gap Region in 28S rRNA Molecules in Brine Shrimp Artemia parthenogenetica and Planarian Dugesia japonica

机译：卤水虾对虾孤雌生殖和日本扁Plan的28S rRNA分子间隙区域的分子特征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In most insects and some other protostomes, a small stretch of nucleotides can be removed from mature 28 S rRNA molecules, which could create two 28S rRNA subunits (28Sα and 28SP). Thus, during electrophoresis, the rRNA profiles of these organisms may differ significantly from the standard benchmark since the two subunits co-migrate with the 18S rRNA. To understand the structure and mechanism of the atypical 28S rRNA molecule, partial fragments of 28Sa and 28Sp in brine shrimp Artemia parthenogenetica and planarian Dugesia japonica were cloned using a modified technology based on terminal transferase. Alignment with the corresponding sequences of 28S rDNAs indicates that there are 41 nucleotides in A parthenogenetica and 42 nucleotides in D. japonica absent from the mature rRNAs. The AU content of the gap sequences of D. japonica and A. parthenogenetica is high. Both the gaps may form stem-loop structure. In D. japonica a UAAU cleavage signal is identified in the loop, but it is absent in A. parthenogenetica. Thus, it is proposed that the gap processing of 28S rRNA was a late enzyme-dependent cleavage event in the rRNA maturational process based on the AU rich gap sequence and the formation of the stem-loop structure to expose the processing segment, while the deletion of the gap region would not affect the structure and function of the 28S rRNA molecule.
机译：在大多数昆虫和其他一些原虫中，可以从成熟的28 S rRNA分子中去除一小段核苷酸，这会产生两个28 S rRNA亚基（28Sα和28SP）。因此，在电泳过程中，由于两个亚基与18S rRNA共同迁移，因此这些生物的rRNA图谱可能与标准基准有显着差异。为了了解非典型28S rRNA分子的结构和机制，使用基于末端转移酶的改良技术克隆了卤虾对虾无性生殖和and虫中的28Sa和28Sp的部分片段。与28S rDNA的相应序列的比对表明，成熟rRNA中缺少孤雌生殖A中的41个核苷酸和粳稻中的42个核苷酸。粳稻和单性A稻的缺口序列的AU含量很高。两个间隙都可以形成茎环结构。在粳稻中，在环中鉴定出了UAAU裂解信号，但在孤雌生殖中不存在该信号。因此，有人提出28S rRNA的缺口加工是基于富含AU的缺口序列和形成茎环结构以暴露加工片段，而缺失的rRNA成熟过程中的酶依赖性晚期切割事件。缺口区域的残基不会影响28S rRNA分子的结构和功能。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号