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首页> 外文期刊>Cell biology international. >CR2 units of CR2 complexes are possibly associated with nucleophilic agents through reactive covalent links.
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CR2 units of CR2 complexes are possibly associated with nucleophilic agents through reactive covalent links.

机译：CR2复合物的CR2单元可能通过反应性共价键与亲核试剂缔合。

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摘要

The human complement receptor type 2 (CR2/CD21), a transmembrane glycoprotein, associates with a variety of surface antigens and proteins in the cell membrane. We examined the possibilities that the CR2 units of CR2 complexes are associated through internal covalent links reactive with nucleophilic agents, e.g. H(2)O or methylamine, and that CR2-positive cells process anti-CR2 monoclonal antibodies (MoAbs). Data from immunoblotting and cytofluorimetry with CR2-binding site-specific MoAbs show that: (i) CR2-positive Raji cells release soluble CR2 isoforms into the medium when incubated in phosphate buffered saline; (ii) despite affecting the detection of one soluble CR2 isoform, methylamine treatment of soluble CR2 allows the detection of another of its isoforms; (iii) limited pre-treatment of cells with methylamine reveals a more heterogeneous CR2-positive cell population or enhances the detection of CR2; (iv) cell treatment with CR2-binding site-specific MoAbs enhances the detection of CR2 isoform(s).The data suggest that CR2 is shed mainly as a soluble CR2 complex, in which the CR2 units link covalently and react with nucleophilic agents. Raji cells may process bound fragments (145kDa) that are recognised by and become bound by anti-CR2 MoAb.
机译：2型人类补体受体（CR2 / CD21）是一种跨膜糖蛋白，可与细胞膜中的多种表面抗原和蛋白结合。我们研究了CR2复合物的CR2单元通过与亲核试剂反应的内部共价键缔合的可能性，例如，亲核试剂。 H（2）O或甲胺，并且CR2阳性细胞处理抗CR2单克隆抗体（MoAbs）。来自具有CR2结合位点特异性MoAb的免疫印迹和细胞荧光检测的数据表明：（i）CR2阳性Raji细胞在磷酸盐缓冲液中孵育时，将可溶性CR2亚型释放到培养基中; （ii）尽管影响了一种可溶性CR2亚型的检测，但是甲胺处理可溶性CR2仍可以检测出另一种亚型; （iii）用甲胺对细胞进行有限的预处理可以揭示CR2阳性细胞群的异质性或提高CR2的检测率; （iv）用CR2结合位点特异性MoAb进行细胞处理可增强CR2亚型的检测。数据表明CR2主要以可溶性CR2复合物的形式脱落，其中CR2单元共价连接并与亲核试剂反应。 Raji细胞可以处理被抗CR2 MoAb识别并与之结合的结合片段（145kDa）。

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