Isolation of rat Kupffer cells: a combined methodology for highly purified primary cultures.

Valatas V; Xidakis C; Roumpaki H; Kolios G; Kouroumalis EA

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Isolation of rat Kupffer cells: a combined methodology for highly purified primary cultures.

机译：分离大鼠库普弗细胞：一种用于高度纯化的原代培养物的组合方法。

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We report a four-step procedure that optimizes the methodology for isolation of highly purified rat Kupffer cells (KC). We combined the previously reported techniques of enzymatic tissue treatment, density gradient centrifugation, centrifugal elutriation and selective adherence. ED-2 immunophenotyping and non-specific esterase histochemistry were used for cell identification. This combination resulted in a satisfactorily high yield of 80-100x10(6)KCs per liver, over 95% positive for ED-2 and 98% viable cells. Cultures of isolated KCs were functionally intact and exhibited a concentration and time-dependent LPS-induced TNF-alpha and nitric oxide production.

机译：我们报告了一个四步骤的过程，该过程优化了高纯度大鼠库普弗细胞（KC）分离的方法。我们结合了以前报道的酶组织治疗，密度梯度离心，离心淘析和选择性粘附技术。 ED-2免疫表型和非特异性酯酶组织化学用于细胞鉴定。这种组合产生令人满意的高产量，每个肝脏80-100x10（6）KCs，ED-2阳性95％以上，活细胞98％以上。分离的KCs培养物功能完整，并表现出浓度和时间依赖性LPS诱导的TNF-α和一氧化氮的产生。

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《Cell biology international.》 |2003年第1期|共7页
作者
Valatas V; Xidakis C; Roumpaki H; Kolios G; Kouroumalis EA;
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正文语种 eng
中图分类细胞生物学;
关键词
methodology lt; 2gt; Kupffer Cells; Need for isolation; isolation aspects; Procedures; 枯否氏细胞;

机译：methodology 2;Kupffer Cells;Need for isolation;isolation aspects;Procedures;枯否氏细胞;

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1. Isolation of rat Kupffer cells: a combined methodology for highly purified primary cultures. [J] . Valatas V, Xidakis C, Roumpaki H, Cell biology international. . 2003,第1期

机译：分离大鼠库普弗细胞：一种用于高度纯化的原代培养物的组合方法。
2. Isolation and primary culture of rat Kupffer cells. [J] . Olynyk JK, Clarke SL Journal of gastroenterology and hepatology . 1998,第8期

机译：大鼠库普弗细胞的分离和原代培养。
3. Preparative free‐flow electrophoresis, a versatile technology complementing gradient centrifugation in the isolation of highly purified cell organelles [J] . Islinger Markus, Wildgruber Robert, V?lkl Alfred Electrophoresis: The Official Journal of the International Electrophoresis Society . 2018,第18期

机译：制备自由流动电泳，通过高度纯化的细胞机分离互补梯度离心的多功能技术
4. DIFFERENTIAL RECOGNITION OF MANNOSYLATED AND FUCOSYLATED LIGANDS BY RAT LIVER ENDOTHELIAL CELLS AND KUPFFER CELLS [C] . Yuriko Hiquchi, Kenji Hyoudou, Makiya Nishikawa, Proceedings of the 29th Annual Meeting of the Controlled Release Society . 2002

机译：大鼠肝内皮细胞和库普弗细胞对甘露糖基化和岩藻糖基化糖的差异识别
5. Prevention of Respiratory Syncytial Virus Attachment Protein Cleavage in Vero Cells Rescues Infectivity of Progeny Virions for Primary Human Airway Cultures. [D] . Corry, Jacqueline D. 2015

机译：预防Vero细胞中呼吸道合胞病毒附着蛋白的切割可拯救子代病毒对原代人气道培养物的感染性。
6. Semiautomated isolation and molecular characterisation of single or highly purified tumour cells from CellSearch enriched blood samples using dielectrophoretic cell sorting [O] . D J E Peeters, B De Laere, G G Van den Eynden, 2013

机译：使用Dielectrophoretic细胞分选从CellSearch富集的血液样本中半自动分离单个或高度纯化的肿瘤细胞并进行分子表征
7. Semiautomated isolation and molecular characterisation of single or highly purified tumour cells from CellSearch enriched blood samples using dielectrophoretic cell sorting [O] . Peeters, D J E, De Laere, B, Van den Eynden, G G, 2013

机译：使用Dielectrophoretic细胞分选从CellSearch富集的血液样本中半自动分离单个或高度纯化的肿瘤细胞并进行分子表征
8. Growth of Plant Cell Cultures. I. Isolation of Cultures, Selection of Media, and Effects of Frequency of Transfer [R] . Mandels, M., Maguire, A., El-Bisi, H. M. 1967

机译：植物细胞培养的生长。 I.培养物的分离，培养基的选择和转移频率的影响

Isolation of rat Kupffer cells: a combined methodology for highly purified primary cultures.

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