• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >Protein profiling and transcript expression levels of heat shock proteins in 17beta-estradiol-treated human MCF-7 breast cancer cells.
【24h】

Protein profiling and transcript expression levels of heat shock proteins in 17beta-estradiol-treated human MCF-7 breast cancer cells.

机译：17β-雌二醇治疗的人MCF-7乳腺癌细胞中热休克蛋白的蛋白谱和转录表达水平。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Using proteomics, proteins regulated by 17beta-estradiol (E2) were identified in human MCF-7 breast cancer cells; 26 proteins including heat shock proteins (Hsps) were differentially regulated by E2 in cells cultured in serum-free condition. When the transcript levels of these proteins and another Hsps were measured by real-time PCR, the transcripts encoding 6 proteins (Hsp56, Hsp90alpha, Hsp110, protein disulfide isomerase related protein, XTP3-transactivated protein A and stathmin 1) were significantly up-regulated and that encoding aminoacylase 1 was down-regulated by E2 in cells cultured with or without serum. The protein profiling and transcript expression patterns of E2-regulated proteins including Hsps in MCF-7 cells suggested the involvement of these proteins in breast carcinogenesis. Proteins (or transcripts) whose expression pattern is altered by E2 might be potent targets for treating breast cancer patients.
机译：使用蛋白质组学技术，在人MCF-7乳腺癌细胞中鉴定出受17β-雌二醇（E2）调控的蛋白质;在无血清条件下培养的细胞中，E2对包括热休克蛋白（Hsps）在内的26种蛋白进行了差异调节。通过实时PCR测定这些蛋白质和另一种Hsps的转录水平时，编码6种蛋白质（Hsp56，Hsp90alpha，Hsp110，蛋白质二硫键异构酶相关蛋白质，XTP3反式激活的蛋白质A和stathmin 1）的转录物显着上调在有血清或无血清培养的细胞中，E2下调了编码氨酰基酶1的编码。 E2调控蛋白（包括Hsps）在MCF-7细胞中的蛋白谱和转录表达模式表明，这些蛋白参与了乳腺癌的致癌作用。其表达方式被E2改变的蛋白质（或转录本）可能是治疗乳腺癌患者的有效靶标。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号