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首页> 外文期刊>Cell biology international. >Influence of paraformaldehyde and EDAC fixation on the demonstrability of hormones (histamine, endorphin, triiodothyronine) in rat immune cells: an immunocytochemical comparative analysis.
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Influence of paraformaldehyde and EDAC fixation on the demonstrability of hormones (histamine, endorphin, triiodothyronine) in rat immune cells: an immunocytochemical comparative analysis.

机译：多聚甲醛和EDAC固定对大鼠免疫细胞中激素（组胺，内啡肽，三碘甲腺嘌呤）的可证明性的影响：免疫细胞化学比较分析。

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The amount and localization of three hormones (histamine, endorphin and triiodothyronine [T(3)]) was measured in male and female rat peritoneal cells (lymphocytes, mast cells, monocyte-macrophage-granulocyte group [mo-gran]) using flow cytometry as well as confocal microscopy after paraformaldehyde (PFA) or EDAC fixation. In the EDAC fixed lymphocytes and mo-gran of female animals two-magnitude higher levels of histamine were measured after EDAC fixation and one magitude higher in mast cells. The amount of T(3) was almost four-fold in lymphocytes and 2.5-4-fold in mast cells and mo-gran. Endorphin content was not altered by the type of fixation. In each cell type in males one magnitude higher levels of histamine and T(3) were measured after EDAC fixation and a small, but significant, elevation of endorphin. Confocal microscopy supports the quantitative data. The results show that (1) the fixation with the crosslinking molecule, EDAC, is more suitable for immunocytochemical studies of amino-acid type hormones in immune cells, (2) more histamine and T(3) are present in the immune cells than it was supposed previously when studying PFA-fixed preparations, (3) the estimation of the amount of peptide hormones seems to be accurate after PFA fixation, (4) there is a quantitative difference comparing the results of PFA and EDAC fixation between males and females.
机译：使用流式细胞仪测量雄性和雌性大鼠腹膜细胞（淋巴细胞，肥大细胞，单核巨噬细胞-粒细胞群[mo-gran]）中三种激素（组胺，内啡肽和三碘甲状腺氨酸[T（3）]）的含量和定位以及低聚甲醛（PFA）或EDAC固定后的共聚焦显微镜。在雌性动物的EDAC固定淋巴细胞和mo-gran中，经EDAC固定后测得的组胺水平升高了2倍，肥大细胞中的组胺水平升高了1倍。 T（3）的量在淋巴细胞中几乎是四倍，在肥大细胞和莫格兰中是2.5-4倍。内啡肽含量不会因固定类型而改变。在EDAC固定后，雄性中每种细胞类型的组胺和T（3）含量都升高了一个数量级，并且内啡肽含量虽小但显着升高。共聚焦显微镜支持定量数据。结果表明：（1）用交联分子EDAC固定更适合于免疫细胞中氨基酸型激素的免疫细胞化学研究，（2）免疫细胞中存在的组胺和T（3）比其多以前是在研究固定PFA的制剂时假设的，（3）固定PFA后估算肽激素的量似乎是准确的，（4）男性和女性之间比较PFA和EDAC固定的结果存在定量差异。

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