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首页> 外文期刊>Cell biology international. >Development of monoclonal antibodies specific to urochordate intracellular epitopes.
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Development of monoclonal antibodies specific to urochordate intracellular epitopes.

机译：对urochordate细胞内表位具有特异性的单克隆抗体的开发。

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Sixteen monoclonal antibodies (MAbs) specific to 2 urochordate genera (Botryllus schlosseri and Botrylloides) intracellular epitopes were generated in mice immunized with a mixture of fresh and paraformaldehyde-fixed cells obtained from animal's blood and cells from dissociated organs. Hybridoma clones were selected by ELISA tests and immunohistochemistry assays on paraffin-embedded animal tissues. Five MAbs were tested for reactions with different zooidal organs and cell compartments; 7 MAbs were tested, separately, on 5 different botryllid colonies (3 Botryllus and 2 Botrylloides). The results revealed high polymorphism. Whereas some of the MAbs recognized, specifically, only part of the botryllid genotypes tested, others recognized only part of the cellular compartments. These MAbs will be used as an important tool in the study of botryllid ascidian immunology and developmental biology, revealing the first wide panel of MAbs specific to urochordate intracellular antigens.
机译：在用新鲜的和多聚甲醛固定的，从动物血液中获得的细胞和游离器官的细胞的混合物进行免疫的小鼠中，产生了两种对urochordate属（Botryllus schlosseri和Botrylloides）细胞内表位具有特异性的十六种单克隆抗体（MAb）。通过石蜡包埋的动物组织的ELISA测试和免疫组织化学分析选择杂交瘤细胞克隆。测试了五个单克隆抗体与不同的人畜共患器官和细胞室的反应;在5个不同的葡萄孢菌落（3个Botryllus和2个Botrylloides）上分别测试了7个单克隆抗体。结果显示高多态性。尽管某些单克隆抗体只能识别出部分测试的葡萄球菌基因型，而另一些单克隆抗体则只能识别出部分的细胞室。这些单克隆抗体将被用作Botryllid孢子虫免疫学和发育生物学研究的重要工具，揭示了最早针对Urochordate细胞内抗原的一系列单克隆抗体。

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