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首页> 外文期刊>Cell biology international. >A GFP trap study uncovers the functions of Gilgamesh protein kinase in Drosophila melanogaster spermatogenesis.
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A GFP trap study uncovers the functions of Gilgamesh protein kinase in Drosophila melanogaster spermatogenesis.

机译：一项GFP捕集器研究揭示了吉尔伽美什蛋白激酶在果蝇果蝇精子发生中的功能。

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摘要

The function of the gene gilgamesh (89B9-12) encoding a casein kinase in Drosophila spermatogenesis was studied. The chimeric Gilgamesh-GFP protein in spermatocytes is cortically located. In the polar and apolar spermatocytes, it concentrates at the terminal ends of the fusome, the organelle that passes through the system of ring canals of the spermatocyte cyst. At the stage of spermatid elongation, the protein associates with the nucleus. A spot of the highest Gilgamesh-GFP concentration in the nucleus co-localizes with gamma-tubulin in the basal body. At later stages, Gilgamesh is localized to the individualization complex (IC), leaving the nuclei somewhat before the IC investment cones, as detected by actin binding. The sterile mutation due to the gilgamesh gene leads to the phenotype of scattered nuclei and altered structure of actin cones in the individualizing spermatid cyst. Ultrastructural evidence confirmed defective spermatid individualization due to the mutation. The phylogenetic origin of the protein, and the connection between vesicular trafficking and spermatid individualization, are discussed.
机译：研究了编码酪蛋白激酶的吉尔伽美什（89B9-12）基因在果蝇生精中的功能。精子细胞中嵌合的吉尔伽美什-GFP蛋白位于皮质。在极性和非极性的精母细胞中，它集中在通过小精囊囊环管系统的细胞器的末端。在精子伸长阶段，蛋白质与细胞核结合。细胞核中最高的吉尔伽美什（Gilgamesh）-GFP浓度斑点与基体中的γ-微管蛋白共定位。在后期，吉尔伽美什（Gilgamesh）被定位在个体化复合体（IC）上，如肌动蛋白结合所检测到的那样，将核保留在IC投资锥的前面。由于吉尔伽美什基因而导致的不育突变导致散在的核表型和个体化精子囊肿中肌动蛋白视锥结构的改变。超微结构证据证实，由于突变，精子个体化缺陷。讨论了该蛋白质的系统发生起源，以及水泡运输和精子个体化之间的联系。

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