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首页> 外文期刊>Cell biology international. >JNK-binding protein 1 regulates NF-kappaB activation through TRAF2 and TAK1.
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JNK-binding protein 1 regulates NF-kappaB activation through TRAF2 and TAK1.

机译：JNK结合蛋白1通过TRAF2和TAK1调节NF-κB的活化。

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摘要

The mitogen-activated protein kinase (MAPK) cascades, including c-Jun N-terminal kinase (JNK), are composed of a MAPK, MAPK kinase (MAPKK), and MAPKK kinase (MAPKKK). Previously, we reported that JNK-binding protein 1 (JNKBP1) enhances JNK activation induced by the TGF-beta-activated kinase1 (TAK1) MAPKKK in transfected cells. We have investigated whether JNKBP1 functions as an adaptor protein for nuclear factor (NF)-kappaB activation mediated by TAK1 in COS-7 cells. Co-expression experiments showed that JNKBP1 interacted with not only TAK1, but also with its upstream regulators, TNF-receptor associated factors 2 and 6 (TRAF2 and TRAF6). An endogenous interaction between JNKBP1 and TRAF2 or TAK1 was confirmed by immunoprecipitation analysis. We also found that JNKBP1 could enhance the NF-kappaB activation induced by TAK1 and TRAF2, and could promote TRAF2 polyubiquitination. These results suggest a scaffolding role for JNKBP1 in the TRAF2-TAK1-NF-kappaB signaling pathway.
机译：丝裂原激活的蛋白激酶（MAPK）级联，包括c-Jun N端激酶（JNK），由MAPK，MAPK激酶（MAPKK）和MAPKK激酶（MAPKKK）组成。以前，我们报道了JNK结合蛋白1（JNKBP1）增强了TGF-β激活的激酶1（TAK1）MAPKKK在转染细胞中诱导的JNK激活。我们已经研究了JNKBP1是否充当COS-7细胞中TAK1介导的核因子（NF）-kappaB激活的衔接蛋白。共表达实验表明，JNKBP1不仅与TAK1相互作用，而且还与其上游调节剂TNF受体相关因子2和6（TRAF2和TRAF6）相互作用。通过免疫沉淀分析确认了JNKBP1和TRAF2或TAK1之间的内源性相互作用。我们还发现，JNKBP1可以增强TAK1和TRAF2诱导的NF-κB活化，并可以促进TRAF2多聚泛素化。这些结果表明JNKBP1在TRAF2-TAK1-NF-kappaB信号通路中的脚手架作用。

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