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首页> 外文期刊>Cell biology international. >1α,25-dihydroxyvitamin D? enhances fast-myosin heavy chain expression in differentiated C2C12 myoblasts.
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1α,25-dihydroxyvitamin D? enhances fast-myosin heavy chain expression in differentiated C2C12 myoblasts.

机译：1α，25-二羟基维生素D？增强分化的C2C12成肌细胞中快速肌球蛋白重链的表达。

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摘要

We investigated the effect of VD3 (1α,25-dihydroxyvitamin D3) on the proliferating, differentiating and differentiated phases of C2C12 myoblasts, a mouse skeletal muscle cell line. VD3 treatment in 10% FBS (fetal bovine serum) inhibited the proliferation and viability of the cells in a dose-dependent manner. It also dose-dependently increased the percentage of cells in the G0/G1 phase as shown by flow cytometry. In the differentiating phase, VD3 treatment inhibited the formation of myotubes and the expression of total myosin heavy chain at both the mRNA and protein levels. In the differentiated phase, treatment had no significant effect on the amount of total myosin heavy chain, as Western blot analysis with MF20 antibody [DSHB (Developmental Studies Hybridoma Bank)] showed. However, significantly greater expression of fast myosin heavy chain in 1 nM VD3 was found by Western blot analysis with MY-32 (Sigma). Thus VD3 inhibited the proliferation of myoblasts during proliferating and differentiating phases, whereas it increased the expression of the fast myosin heavy chain isoform in the differentiated phase. The data indicate that an adequate concentration of VD3 might have an anabolic effect on differentiated skeletal muscle.
机译：我们研究了VD3（1α，25-二羟基维生素D3）对C2C12成肌细胞（小鼠骨骼肌细胞系）增殖，分化和分化期的影响。在10％FBS（胎牛血清）中进行VD3处理以剂量依赖的方式抑制了细胞的增殖和活力。如流式细胞术所示，它还剂量依赖性地增加了G0 / G1期细胞的百分比。在分化阶段，VD3处理在mRNA和蛋白质水平上均抑制了肌管的形成和总肌球蛋白重链的表达。正如在MF20抗体[DSHB（Developmental Studies Hybridoma Bank）]进行的蛋白质印迹分析所显示的，在分化期，治疗对总肌球蛋白重链的量没有显着影响。然而，通过MY-32（Sigma）的蛋白质印迹分析发现，快速肌球蛋白重链在1 nM VD3中的表达明显更高。因此，VD3在增殖和分化阶段抑制成肌细胞的增殖，而在分化期增加快速肌球蛋白重链同工型的表达。数据表明适当浓度的VD3可能对分化的骨骼肌有合成代谢作用。

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