...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >Stimulatory effect of HGF-overexpressing adipose tissue-derived mesenchymal stem cells on thymus regeneration in a rat thymus involution model
【24h】

Stimulatory effect of HGF-overexpressing adipose tissue-derived mesenchymal stem cells on thymus regeneration in a rat thymus involution model

机译：HGF过量表达的脂肪组织间充质干细胞对大鼠胸腺退化模型中胸腺再生的刺激作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The thymus is the central lymphoid organ providing a unique and essential microenvironment for T-cell precursor development into mature functionally competent T-lymphocytes. Thus, it is important to develop the strategies for enhancing thymic regeneration from involution induced by a variety of clinical treatments and conditions. Hepatocyte growth factor (HGF) promotes proliferation in a variety of cell types. We have used stem cell-based HGF gene therapy to enhance regeneration from acute thymic involution. HGF-overexpressing human adipose tissue-derived mesenchymal stem cells (HGF-hATMSCs) were generated by liposomal transfection with the pMEX expression vector, constructed by inserting the HGF gene. Significantly increased HGF expression in these cells was confirmed by reverse transcription-polymerase chain reaction and an enzyme-linked immunosorbent assay. HGF produced by HGF-hATMSCs enhanced the proliferation of a mouse thymic epithelial cell line and the expression of interleukin-7 in vitro. We also examined the effect of HGF-hATMSCs on thymic regeneration in rats with acute thymic involution. Significant increases in thymus size and weight, as well as the number of thymocytes (especially, early thymocyte progenitors), were seen in the HGF-hATMSCs-treated rats compared to saline-treated control animals. A stimulatory effect of HGF-hATMSCs on thymic regeneration has therefore been shown, highlighting the clinical value of HGF-hATMSCs for treating thymic involution.
机译：胸腺是中央淋巴器官，为T细胞前体发育成功能成熟的T淋巴细胞提供了独特而必要的微环境。因此，重要的是开发增强由多种临床治疗方法和条件引起的退化引起的胸腺再生的策略。肝细胞生长因子（HGF）促进多种细胞类型的增殖。我们已经使用基于干细胞的HGF基因疗法来增强急性胸腺退化的再生。通过脂质体转染pMEX表达载体（通过插入HGF基因构建），可产生HGF过表达的人脂肪组织来源的间充质干细胞（HGF-hATMSC）。通过逆转录-聚合酶链反应和酶联免疫吸附试验证实了这些细胞中HGF的表达明显增加。 HGF-hATMSCs产生的HGF在体外增强了小鼠胸腺上皮细胞系的增殖和白介素7的表达。我们还检查了HGF-hATMSCs对急性胸腺退化的大鼠胸腺再生的影响。与盐水处理的对照动物相比，在HGF-hATMSCs处理的大鼠中观察到胸腺大小和重量以及胸腺细胞（特别是早期胸腺细胞祖细胞）的数量显着增加。因此，已经显示出HGF-hATMSC对胸腺再生的刺激作用，突出了HGF-hATMSC在治疗胸腺退化中的临床价值。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号