• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >3-D structure, volume, and DNA content of erythrocyte nuclei of polyploid fish
【24h】

3-D structure, volume, and DNA content of erythrocyte nuclei of polyploid fish

机译：多倍体鱼类红细胞核的3-D结构，体积和DNA含量

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have explored the potential relationship between ploidy level, DNA content (pg DNAucleus), and dimensional characteristics, such as volume (μm3), surface area (μm2), and 3-D structure of erythrocyte nuclei in a series of fish ploidy level models using Feulgen image analysis densitometry, flow cytometry, and confocal laser scanning microscopy. The species were diploid tench (Tinca tinca) (2n), Cuban gar (Atractosteus tristoechus) (2n), triploid tench (3n), evolutionary tetraploid sterlet (Acipenser ruthenus) (4n), evolutionary octaploid Siberian sturgeon (A. baerii) (8n), triploid Siberian sturgeon exhibiting dodecaploidy (12n), evolutionary 12n shortnose sturgeon (A. brevirostrum), and experimentally obtained sturgeon hybrids that were tetraploid, hexaploid (6n), heptaploid (7n), octaploid, decaploid (10n), dodecaploid and/or tetradecaploid (14n). Increase in ploidy was accompanied by growth of the nucleus and an increase in the number of flattened ellipsoid nuclei with increased transverse diameter. The volume (Vvoxel) of erythrocyte nuclei, as the sum of voxels calculated from live cells, seems more accurate than volume(Vaxis) calculated frommeasuring the major and minor axis, especially at higher and odd ploidy levels. Data of absolute and relative DNA content were in agreement with previously published reports. Species of the same ploidy level, but differing in DNA content, had a similar mean erythrocyte nuclear volume (Vvoxel), as demonstrated in sterlet and a hybrid of sterlet and beluga (48.3 and 48.9μm3, respectively), with a respective mean DNA content of 3.74 and 3.10 pg DNAucleus. A similar relationship was found for the ploidy 6n, 10n, 12n. The surface-to-volume ratio decreased non-linearly with increasing ploidy. The DNA in erythrocyte nuclei appeared to be more densely packed with increase in ploidy level.
机译：我们探索了一系列鱼倍性中倍性水平，DNA含量（pg DNA /核）和尺寸特征（如体积（μm3），表面积（μm2）和红细胞核的3-D结构）之间的潜在关系。使用Feulgen图像分析光密度法，流式细胞仪和共聚焦激光扫描显微镜的水平模型。该物种是二倍体ten（Tinca tinca）（2n），古巴（Atractosteus tristoechus）（2n），三倍体ten（3n），进化四倍体小实蝇（Acipenser ruthenus）（4n），进化八倍体西伯利亚st（A. baerii）（ 8n），三倍体西伯利亚st显示十二倍体（12n），进化12n短鼻st（A. brevirostrum），并通过实验获得的st鱼杂种为四倍体，六倍体（6n），七倍体（7n），八倍体，十倍体（10n），十二倍体和/或四倍体（14n）。倍性的增加伴随着核的增长和扁平椭圆形核的数量随横向直径的增加而增加。作为从活细胞计算的体素的总和，红细胞核的体积（Vvoxel）似乎比从测量长轴和短轴所计算的体积（Vaxis）更为准确，尤其是在较高和奇数倍性水平下。绝对和相对DNA含量的数据与以前发表的报告一致。相同倍性水平但DNA含量不同的物种具有相似的平均红细胞核体积（Vvoxel），如在sterlet以及sterlet和beluga的杂种中所显示的（分别为48.3和48.9μm3），分别具有平均DNA含量3.74和3.10 pg DNA /核。对于倍性6n，10n，12n也发现了类似的关系。表面积与体积的比例随倍性的增加呈非线性下降。红细胞核中的DNA似乎更紧密堆积，倍性水平增加。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号