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首页> 外文期刊>Cell biology international. >KCl stimulation and ultrastructural responses of tannic acid-stained cholinergic synaptic terminals.
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KCl stimulation and ultrastructural responses of tannic acid-stained cholinergic synaptic terminals.

机译：单宁酸染色的胆碱能突触末端的KCl刺激和超微结构反应。

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The quantal-vesicular hypothesis equates miniature end-plate potentials (MEPPs) with fusions of synaptic vesicles. MEPP production thus predicts vesicle losses, increases in vesicle fusions and increases in terminal plasma membrane. MEPP production and these ultrastructural parameters have been evaluated in the cholinergic presynaptic terminals of skate electric organ following tannic acid saline incubation, known to promote capture and selective staining of dense-core granule fusions, and KCl stimulation, known to elevate MEPP production dramatically in these cholinergic terminals. After pretreatment in tannic acid-elasmobranch saline, KCl stimulation produced MEPPs at 40/s/microm(2)of terminal surface for several minutes with gradual reduction to spontaneous levels by 25-30 min. No loss of vesicles, no vesicle fusions, no expansions of plasma membrane and no tannic acid enhanced staining of vesicles or vacuoles accompanied the generation of 800 MEPPs/microm(3)of terminals having densities of 567 vesicles/microm(3). No ultrastructural footprints were found to support the notion that unnaturally high rates of vesicular exocytosis had occurred. Copyright 2000 Academic Press.
机译：数量囊泡假说等于微型终板电位（MEPPs）与突触囊泡的融合。因此，MEPP的产生预测了囊泡的损失，囊泡融合的增加以及终末质膜的增加。单宁酸盐水温育后，已知在滑冰电器官的胆碱能突触前末端中评估了MEPP的产生和这些超微结构参数，已知这些物质可促进致密颗粒融合物的捕获和选择性染色，而KCl刺激可显着提高MEPP的产生。胆碱能的终端。在鞣酸-弹枝盐水中进行预处理后，KCl刺激以40 / s / microm（2）的末端表面生成MEPPs数分钟，并在25-30分钟内逐渐降低至自发水平。没有囊泡损失，没有囊泡融合，没有质膜扩张，没有鞣酸增强了囊泡或液泡的染色，伴随着800 MEPPs / microm（3）末端的产生，密度为567囊泡/ microm（3）。没有发现超微结构的足迹支持以下观点，即发生了异常高的囊泡胞吐率。版权所有2000学术出版社。

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