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首页> 外文期刊>Cell biology international. >Effects of the myosin inhibitor 2,3-butanedione monoxime (BDM) on cell shape, locomotion and fluid pinocytosis in human polymorphonuclear leucocytes.
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Effects of the myosin inhibitor 2,3-butanedione monoxime (BDM) on cell shape, locomotion and fluid pinocytosis in human polymorphonuclear leucocytes.

机译：肌球蛋白抑制剂2,3-丁二酮单肟（BDM）对人多形核白细胞的细胞形状，运动和液体胞吞作用的影响。

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摘要

We investigated the role of myosin in polymorphonuclear leucocyte (PMN) shape changes, locomotion, and fluid pinocytosis using the myosin inhibitor 2,3 butanedione monoxime (BDM). Treatment of resting spherical PMNs with BDM produced spheroid cells showing small continuous shape changes (IC(50)=15.5 m m BDM) and occasionally small blebs. Cell polarity, as induced by the chemotactic peptide fNLPNTL or by colchicine, and locomotion were completely suppressed (IC(50)=8.4 to 10 m m). Suppression of fNLPNTL- or colchicine-induced cell polarity produced spheroid cells, suppression of PMA-induced shape changes and fluid pinocytosis produced non-motile spherical cells (IC(50)=25 to 30 m m BDM). BDM suppressed formation of lamellipodia but not formation of blebs. Suppression of microvilli by BDM as observed in resting spherical cells was partially antagonized by PMA. The results suggest that myosin is involved in stabilizing the shape of resting spherical cells, including microvilli, and that myosin is required for cell polarity, locomotion, fluid pinocytosis and for formation of lamellipodia, but not for formation of blebs. Copyright 2000 Academic Press.
机译：我们调查了肌球蛋白在多形核白细胞（PMN）的形状变化，运动和流体胞吞作用中的作用，使用的是肌球蛋白抑制剂2,3丁二酮单肟（BDM）。用BDM处理静止的球形PMN产生的球状细胞显示出小的连续形状变化（IC（50）= 15.5 m m BDM），偶尔有小气泡。由趋化肽fNLPNTL或秋水仙碱诱导的细胞极性和运动被完全抑制（IC（50）= 8.4至10 m m）。抑制fNLPNTL或秋水仙碱诱导的细胞极性可产生球状细胞，抑制PMA诱导的形状变化和液体胞饮作用可产生非活动性球状细胞（IC（50）= 25至30 m m BDM）。 BDM抑制了片状脂蛋白的形成，但抑制了气泡的形成。在静止的球形细胞中观察到的BDM对微绒毛的抑制作用被PMA部分拮抗。结果表明，肌球蛋白参与稳定包括微绒毛在内的静止球形细胞的形状，并且肌球蛋白是细胞极性，运动，体液性红细胞增多症和层状脂质体形成所必需的，但不是泡的形成所必需的。版权所有2000学术出版社。

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