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首页> 外文期刊>Cell biology international. >Are cells rescued from 'low density death' by co-operation between phospholipases C AND D?
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Are cells rescued from 'low density death' by co-operation between phospholipases C AND D?

机译：是否通过磷脂酶C和D之间的合作将细胞从“低密度死亡”中拯救出来？

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摘要

Cells of the ciliate Tetrahymena thermophila die when transferred at low density to a lipid-free nutritionally complete medium. This death is prevented and they will start to proliferate if protein kinase C is activated and this activation is sustained. We propose that this takes place in two stages. Firstly, the phospholipase C pathway beginning with and specific for phosphatidylinositol leads to the formation of diacylglycerol and inositol tris -phosphate. Diacylglycerol activates protein kinase C, and inositol tris -phosphate via Ca(2+)phospholipase D (PLD). Secondly, the protein kinase C response can now be sustained by diacylglycerol produced by phospholipase D, using phosphatidylcholine and phosphatidylserine as substrates. Should this switching from PI-specific phospholipase C (PLC) to phospholipase D fail, then the cell will die in the course of milliseconds during the minutes following inoculation. Copyright 2000 Academic Press.
机译：当纤毛状四膜虫嗜热菌的细胞以低密度转移到无脂营养完全培养基中时会死亡。如果蛋白激酶C被激活并且这种激活得以持续，则可以防止这种死亡，并且它们将开始增殖。我们建议这分两个阶段进行。首先，从磷脂酰肌醇开始并特异于磷脂酰肌醇的磷脂酶C途径导致二酰基甘油和肌醇三磷酸酯的形成。二酰基甘油激活蛋白激酶C和肌醇三磷酸通过Ca（2+）磷脂酶D（PLD）。其次，现在可以使用磷脂酰胆碱和磷脂酰丝氨酸作为底物，通过磷脂酶D产生的二酰基甘油来维持蛋白激酶C反应。如果从PI特异性磷脂酶C（PLC）切换到磷脂酶D失败，则细胞将在接种后的数分钟内死亡。版权所有2000学术出版社。

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