首页> 外文期刊>Cell biology international. >Inhibition of proliferation of contaminating fibroblasts by D-valine in cultures of smooth muscle cells from human myometrium.
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Inhibition of proliferation of contaminating fibroblasts by D-valine in cultures of smooth muscle cells from human myometrium.

机译:D-缬氨酸抑制人子宫肌层平滑肌细胞培养物中污染性成纤维细胞的增殖。

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Replacement of L-valine with D-valine in a standard culture medium can selectively inhibit fibroblast proliferation. The aim of the present study was to investigate whether human myometrial cells cultured with D-valine instead of L-valine can survive and express their characteristics. Cultured cells (95-98%) maintain expression of the intermediate filament desmin, which is the specific marker for mature muscle cells. By transmission electron microscopy, the cells showed the general morphology of smooth muscle cells in culture. Oxytocin in serum-free culture medium at 37 degrees C (5 min) caused a concentration-dependent increase in cellular Na and total Ca, and a decrease in K content as determined by X-ray microanalysis. The percentage of cells cultured with D-valine responding to oxytocin stimulation was larger than that of cells cultured with L-valine, suggesting less contamination of smooth muscle cells by fibroblasts in the presence of D-valine. As shown by measurements with fura-2, D-valine-cultured cells retained the characteristic increase in intracellular free Ca2+ ions after oxytocin stimulation.
机译:在标准培养基中用D-缬氨酸替代L-缬氨酸可以选择性抑制成纤维细胞增殖。本研究的目的是研究用D-缬氨酸而不是L-缬氨酸培养的人肌细胞是否可以存活并表达其特征。培养的细胞(95-98%)保持中间丝结蛋白的表达,这是成熟肌肉细胞的特异性标记。通过透射电子显微镜,细胞显示出培养物中平滑肌细胞的一般形态。通过X射线显微分析确定,无血清培养基中的催产素在37摄氏度(5分钟)时引起细胞Na和总Ca的浓度依赖性增加,而K含量减少。用D-缬氨酸培养的细胞对催产素刺激有反应的百分比大于用L-缬氨酸培养的细胞的百分比,这表明在D-缬氨酸存在下成纤维细胞对平滑肌细胞的污染较少。如通过fura-2的测量结果所示,D-缬氨酸培养的细胞在催产素刺激后保留了细胞内游离Ca2 +离子的特征性增加。

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