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首页> 外文期刊>Cell biology international. >Recombinant human interleukin-8, but not human interleukin-1beta, induces bovine neutrophil migration in an in vitro co-culture system.
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Recombinant human interleukin-8, but not human interleukin-1beta, induces bovine neutrophil migration in an in vitro co-culture system.

机译：重组人白细胞介素8，而不是人白细胞介素1β，在体外共培养系统中诱导牛嗜中性粒细胞迁移。

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摘要

A co-culture system was established by culturing a bovine mammary epithelial cell line (MAC-T) and a bovine aortic endothelial cell line on calf tail collagen pre-coated inserts. This system allowed us to study bovine neutrophil migration across endothelium, extracellular matrix (ECM), and epithelium in the correct sequence and direction in vitro. The effect of recombinant interleukin-1beta (rHIL-1beta) and interleukin-8 (rHIL-8) on bovine neutrophil migration was investigated using this system. rHIL-8 stimulated bovine neutrophil migration in a dose-dependent fashion. The level of migrating bovine neutrophils increased up to approximately 25% when 100 ng/ml of rHIL-8 was used. On the other hand, rHIL-1beta at concentrations up to 100 ng/ml did not directly induce bovine neutrophil migration. Furthermore, pre-incubation with 5 ng/ml of rHIL-1beta in the co-culture system for 4 or 24 h failed to have any effect. These results suggest that IL-8 plays an important role in neutrophil migration into bovine mammary glands during mastitis. Copyright 2000 Academic Press.
机译：通过在小腿尾胶原预涂插入物上培养牛乳腺上皮细胞系（MAC-T）和牛主动脉内皮细胞系来建立共培养系统。该系统使我们能够在体外以正确的顺序和方向研究牛嗜中性粒细胞跨内皮，细胞外基质（ECM）和上皮细胞的迁移。使用该系统研究了重组白介素-1β（rHIL-1beta）和白介素8（rHIL-8）对牛中性粒细胞迁移的影响。 rHIL-8以剂量依赖性方式刺激牛中性粒细胞迁移。当使用100 ng / ml rHIL-8时，迁移的牛中性粒细胞水平增加至约25％。另一方面，浓度高达100 ng / ml的rHIL-1beta不会直接诱导牛嗜中性粒细胞迁移。此外，在共培养系统中与5 ng / ml的rHIL-1beta预温育4或24 h无法产生任何效果。这些结果表明IL-8在乳腺炎期间在嗜中性粒细胞向牛乳腺的迁移中起重要作用。版权所有2000学术出版社。

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