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首页> 外文期刊>Legume research >Specific activities and transcript levels of glycolytic enzymes under dehydration in chickpea (Cicer arietinum L.) seedlings
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Specific activities and transcript levels of glycolytic enzymes under dehydration in chickpea (Cicer arietinum L.) seedlings

机译：鹰嘴豆（Cicer arietinum L.）幼苗脱水过程中糖酵解酶的比活和转录水平

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摘要

The effect of dehydration on the specific activities and transcript levels of several glycolytic enzymes was determined in two chickpea (Cicer arietinwn L.) cultivars- Pusa362 (drought tolerant) and SBD377 (drought sensitive). Dehydration treatment was imposed on 6 days old chickpea seedlings by removing them from pots and keeping them on table top on 3MM Whatman paper at room temperature for 5 h. The stress imposed was judged by determining relative water content (RWC) and proline content. RWC decreased and proline content accumulated after five hours of dehydration treatment. The decline in RWC was higher in SBD377 than in Pusa362, while varietal differences were absent in proline content. With the exception ofNADP-glyceraldehyde-3-phospahte dehydrogense, whose activity as well as transcript level showed marginal decrease in both the varieties, dehydration had little effect on all glycolytic enzymes studied.
机译：在两个鹰嘴豆（Cicer arietinwn L.）栽培品种-Pusa362（耐干旱）和SBD377（干旱敏感）中确定了脱水对几种糖酵解酶比活性和转录水平的影响。对六天大的鹰嘴豆幼苗进行脱水处理，方法是将它们从盆中取出，并在室温下将其放在3MM Whatman纸上的桌面上保持5小时。通过确定相对水含量（RWC）和脯氨酸含量来判断施加的压力。脱水处理五小时后，RWC下降，脯氨酸含量累积。 SBD377的RWC下降高于Pusa362的下降，脯氨酸含量不存在品种差异。除了NADP-甘油醛-3-磷酸脱氢酶的活性和转录水平均在两个品种中均略有下降外，脱水对所有研究的糖酵解酶影响均很小。

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