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首页> 外文期刊>Laser Focus World: The Magazine for the Photonics & Optoelectronics Industry >FLUORESCENCE IMAGING: Optical filters optimize laser-based fluorescence imaging systems
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FLUORESCENCE IMAGING: Optical filters optimize laser-based fluorescence imaging systems

机译：荧光成像：滤光片可优化基于激光的荧光成像系统

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摘要

The advent of lasers as fluorescence light sources imposes new constraints and demands on the optical filters required for optimal operation of these laser-based imaging systems. Because of their high brightness, stability, longevity, and narrow spectral bandwidth, lasers have been advantageously replacing conventional broadband light sources for fluorescence imaging applications. In addition to allowing higher-sensitivity visualization and enhanced throughput in imaging applications, several unique properties of lasers--narrow beam divergence, high degree of spatial and temporal coherence, and well-defined polarization properties--have spawned new fluorescence imaging techniques. The advent of lasers as fluorescence light sources, however, imposes new constraints on imaging systems and their components. Optical filters used in laser-based imaging systems, for example, must meet specific unique requirements compared to filters used in broadband-light-source-based instruments.
机译：激光器作为荧光光源的出现对这些基于激光的成像系统的最佳操作所需的滤光片施加了新的限制和要求。由于它们的高亮度，稳定性，寿命和窄光谱带宽，激光器已经有利地替代了用于荧光成像应用的常规宽带光源。除了在成像应用中实现更高灵敏度的可视化和提高吞吐量外，激光器还具有多种独特的特性-窄光束发散，高度的时空相干性和明确定义的偏振特性-催生了新的荧光成像技术。然而，激光作为荧光光源的出现，对成像系统及其组件施加了新的限制。例如，与基于宽带光源的仪器中使用的滤光器相比，基于激光的成像系统中使用的滤光器必须满足特定的独特要求。

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