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首页> 外文期刊>Biochemistry >Localization of Non-native D-Glyceraldehyde-3-Phosphate Dehydrogenase in Growing and Apoptotic HeLa Cells
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Localization of Non-native D-Glyceraldehyde-3-Phosphate Dehydrogenase in Growing and Apoptotic HeLa Cells

机译：非天然D-甘油醛-3-磷酸脱氢酶在生长和凋亡的HeLa细胞中的定位

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摘要

Monoclonal antibodies that could not bind native tetramers of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) but could bind to dimeric, monomeric, or denatured forms of GAPDH were used to investigate its intracellular localization. These antibodies distinctly stained the nucleus in growing HeLa cells. In the cytoplasm, non-native GAPDH was colocalized with actin filaments. Incubation of HeLa cells with tumor necrosis factor a (TNF-α) and the protein synthesis inhibitor emetine led to a drastic increase in the amount of the non-native GAPDH in the nuclei. Overproduction of Bcl-2 protein did not change the non-native GAPDH localization in the growing HeLa cells but prevented the development of apoptosis and the increase in the amount of non-native GAPDH in the nuclei upon incubation with TNF-α.
机译：单克隆抗体不能与3-磷酸甘油醛脱氢酶（GAPDH）的天然四聚体结合，但可以与GAPDH的二聚体，单体或变性形式结合，用于研究其胞内定位。这些抗体明显地使正在生长的HeLa细胞中的细胞核染色。在细胞质中，非天然GAPDH与肌动蛋白丝共定位。 HeLa细胞与肿瘤坏死因子a（TNF-α）和蛋白质合成抑制剂依美丁汀的孵育导致细胞核中非天然GAPDH的数量急剧增加。 Bcl-2蛋白的过量生产不会改变生长的HeLa细胞中非天然GAPDH的定位，但可以防止细胞凋亡的发生以及与TNF-α孵育后细胞核中非天然GAPDH数量的增加。

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