Clinical coherent anti-Stokes Raman scattering and multiphoton tomography of human skin with a femtosecond laser and photonic crystal fiber

Hans Georg Breunig; Martin Weinigel; Rainer Buckle; Marcel Kellner-Hofer; Jurgen Lademann; Maxim E. Darvin; Wolfram Sterry; Karsten Konig

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Clinical coherent anti-Stokes Raman scattering and multiphoton tomography of human skin with a femtosecond laser and photonic crystal fiber

机译：飞秒激光和光子晶体光纤对人皮肤的临床相干抗斯托克斯拉曼散射和多光子层析成像

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We report on in vivo coherent anti-Stokes Raman scattering spectroscopy (CARS), two-photon fluorescence and second-harmonic-generation imaging on human skin with a novel multimodal clinical CARS/multiphoton tomograph. CARS imaging is realized by a combination of femtosecond pulses with broadband continuum pulses generated by a photonic crystal fiber. The images reveal the microscopic distribution of (i) non-fluorescent lipids, (ii) endogenous fluorophores and (iii) the collagen network inside the human skin in vivo with subcellular resolution. Examples of healthy as well as cancer-affected skin are presented.

机译：我们报告了一种新型多模式临床CARS /多光子断层扫描仪在人体皮肤上的体内相干抗斯托克斯拉曼散射光谱（CARS），双光子荧光和二次谐波生成成像。通过飞秒脉冲与光子晶体光纤产生的宽带连续光脉冲的组合来实现CARS成像。图像揭示了（i）非荧光脂质，（ii）内源性荧光团和（iii）人体皮肤在体内具有亚细胞分辨率的胶原网络的微观分布。介绍了健康以及受癌症影响的皮肤的示例。

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《Laser physics letters》 |2013年第2期|共5页
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Hans Georg Breunig; Martin Weinigel; Rainer Buckle; Marcel Kellner-Hofer; Jurgen Lademann; Maxim E. Darvin; Wolfram Sterry; Karsten Konig;
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中图分类激光物理和基本理论;
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1. Clinical coherent anti-Stokes Raman scattering and multiphoton tomography of human skin with a femtosecond laser and photonic crystal fiber [J] . Hans Georg Breunig, Martin Weinigel, Rainer Buckle, Laser physics letters . 2013,第2期

机译：飞秒激光和光子晶体光纤对人皮肤的临床相干抗斯托克斯拉曼散射和多光子层析成像
2. Impact of refractive index mismatches on coherent anti-Stokes Raman scattering and multiphoton autofluorescence tomography of human skin in vivo [J] . Weinigel M., Breunig H. G., Darvin M. E., Physics in medicine and biology. . 2015,第17期

机译：屈光指数不匹配对人体皮肤的相干反斯托克斯拉曼散射和多光荧光荧光层面的影响
3. Time-resolved coherent anti-Stokes Raman scattering with a femtosecond soliton output of a photonic-crystal fiber [J] . Sidorov-Biryukov DA, Serebryannikov EE, Zheltikov AM Optics Letters . 2006,第15期

机译：飞秒孤子输出光子晶体光纤的时间分辨相干反斯托克斯拉曼散射
4. Two-dimensional femtosecond coherent anti-Stokes Raman scattering spectroscopy using a chirped supercontinuum generated from a photonic crystal fiber [C] . Hideaki Kano, Hiro-o Hamaguchi International Conference on Ultrafast Phenomena . 2005

机译：二维飞秒相干抗激光使用光子晶体纤维产生的啁啾超连续的拉曼散射光谱
5. Single-laser-shot femtosecond coherent anti-Stokes Raman scattering temperature measurements at 5 kHz in a jet diffusion flame. [D] . Bangar, Devashish S. 2012

机译：在射流扩散火焰中以5 kHz的单激光飞秒飞秒相干反斯托克斯拉曼散射温度测量值。
6. Coherent anti-Stokes Raman scattering imaging with a laser source delivered by a photonic crystal fiber [O] . Haifeng Wang, Terry B. Huff, Ji-Xin Cheng -1

机译：用光子晶体光纤传输的激光源进行相干反斯托克斯拉曼散射成像
7. Coherent anti-Stokes Raman scattering imaging with a laser source delivered by a photonic crystal fiber [O] . Haifeng Wang, Terry B. Huff, Ji-Xin Cheng 2006

机译：相干的防斯托克斯拉曼散射成像与光子晶纤维输送的激光源

Clinical coherent anti-Stokes Raman scattering and multiphoton tomography of human skin with a femtosecond laser and photonic crystal fiber

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