...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection for Carbohydrate Analysis of Glycoproteins
【24h】

High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection for Carbohydrate Analysis of Glycoproteins

机译：高效阴离子交换色谱-脉冲安培检测糖蛋白的碳水化合物分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

High-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) is an established technique for the carbohydrate analysis of glycoproteins. HPAE-PAD is routinely used for determinations of monosaccharide, sialic acid, mannose-6-phosphate (M-6-P), and oligosaccharide contents of a glycoprotein. This is true for both the initial investigation of a glycoprotein and routine assays of recombinant therapeutic glycoproteins. This contribution reviews the fundamentals of HPAE-PAD, recent technological improvements, and advances in the last ten years in its application to carbohydrate analysis of glycoproteins. The application areas reviewed include monosaccharide determinations, sialic acid determinations, M-6-P determinations, sugar alcohol determinations, analysis of polysialic acids, neutral and charged oligosaccharide analysis, following glycosidase andglycosyltransferase reactions, and coupling HPAE-PAD to mass spectrometry (MS).
机译：带有脉冲安培检测的高性能阴离子交换色谱法（HPAE-PAD）是一种用于糖蛋白碳水化合物分析的成熟技术。 HPAE-PAD通常用于测定糖蛋白的单糖，唾液酸，6-磷酸甘露糖（M-6-P）和寡糖含量。对于糖蛋白的初步研究和重组治疗性糖蛋白的常规测定都是如此。本文对HPAE-PAD的基本原理，最新的技术改进以及最近十年在糖蛋白碳水化合物分析中的应用进行了回顾。审查的应用领域包括单糖测定，唾液酸测定，M-6-P测定，糖醇测定，多唾液酸分析，中性和带电荷的低聚糖分析（糖苷酶和糖基转移酶反应之后）以及将HPAE-PAD与质谱（MS）偶联。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号