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首页> 外文期刊>Cell motility and the cytoskeleton >Role of the Na+/Ca2+ exchanger in calcium homeostasis and human sperm motility regulation.
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Role of the Na+/Ca2+ exchanger in calcium homeostasis and human sperm motility regulation.

机译：Na + / Ca2 +交换剂在钙稳态和人类精子运动调节中的作用。

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摘要

A number of cell functions, such as flagellar beating, swimming velocity, acrosome reaction, etc., are triggered by a Ca2+ influx across the cell membrane. For appropriate physiological functions, the motile human sperm maintains the intracellular free calcium concentration ([Ca2+]i) at a submicromolar level. The objective of this study was to determine the role of the Na+/Ca2+ exchanger (NCX) in the maintenance of [Ca2+]i in human spermatozoa. Spermatozoa maintained in extracellular medium containing>or=1 microM Ca2+ exhibited motility similar to that of the control. In addition to several calcium transport mechanisms described earlier, we provide evidence that the NCX plays a crucial role in the maintenance of [Ca2+]i. Three chemically unrelated inhibitors of the NCX (bepridil, DCB (3',4'-dichlorobenzamil hydrochloride), and KB-R7943) all blocked human sperm motility in a dose and incubation time dependent manner. The IC50 values for bepridil, DCB, and KB-R7943 were 16.2, 9.8, and 5.3 microM, respectively. The treatment with the above-mentioned blockers resulted in an elevated [Ca2+]i and a decreased [Na+]i. The store-operated calcium channel (SOCC) inhibitor SKF 96365 also blocked the sperm motility (IC50=2.44 microM). The presence of the NCX antigen in the human spermatozoa was proven by flow cytometry, confocal laser scanning microscopy, and immunoblotting techniques. Calcium homeostasis of human spermatozoa is maintained by several transport proteins among which the SOCC and the NCX may play a major role.
机译：Ca2 +流入细胞膜会触发许多细胞功能，例如鞭毛跳动，游泳速度，顶体反应等。对于适当的生理功能，运动的人类精子将细胞内游离钙浓度（[Ca2 +] i）维持在亚微摩尔水平。这项研究的目的是确定Na + / Ca2 +交换剂（NCX）在维持人类精子中[Ca2 +] i的作用。维持在含有>或= 1 microM Ca2 +的细胞外培养基中的精子表现出与对照相似的运动能力。除了前面描述的几种钙转运机制外，我们提供的证据表明NCX在[Ca2 +] i的维持中起着至关重要的作用。三种化学上不相关的NCX抑制剂（贝普地尔，DCB（3'，4'-二氯苯甲酰盐酸盐）和KB-R7943）均以剂量和温育时间依赖性方式阻断人的精子运动。贝普地尔，DCB和KB-R7943的IC50值分别为16.2、9.8和5.3 microM。用上述阻断剂治疗导致[Ca 2+] i升高和[Na +] i降低。钙池操纵的钙离子通道（SOCC）抑制剂SKF 96365也阻断了精子运动（IC50 = 2.44 microM）。通过流式细胞术，共聚焦激光扫描显微镜和免疫印迹技术证明了人类精子中NCX抗原的存在。人精子的钙稳态是由几种转运蛋白维持的，其中SOCC和NCX可能起主要作用。

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