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首页> 外文期刊>Cell motility and the cytoskeleton >Jupiter, a new Drosophila protein associated with microtubules.
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Jupiter, a new Drosophila protein associated with microtubules.

机译：木星，一种新的果蝇蛋白，与微管相关。

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摘要

In this study we describe a novel Drosophila protein Jupiter, which shares properties with several structural microtubule-associated proteins (MAPs) including TAU, MAP2, MAP4. Jupiter is a soluble unfolded molecule with the high net positive charge, rich in Glycine. It possesses two degenerated repeats around the sequence PPGG, separated by a Serine-rich region. Jupiter associates with microtubules in vitro and, fused with the green fluorescent protein (GFP), is an excellent marker to follow microtubule dynamics in vivo. In a jupiter transgenic Drosophila strain generated by the "protein-trap" technique, Jupiter:GFP fusion protein localizes to the microtubule network through the cell cycle at the different stages of development. We found particularly high Jupiter:GFP concentrations in the young embryo, larval nervous system, precursors of eye photoreceptors and adult ovary. Moreover, from jupiter:gfp embryos we have established two permanent cell lines presenting strongly fluorescent microtubules during the whole cell cycle. In these cells, the distribution of the Jupiter:GFP fusion protein reproduces microtubule behavior upon treatment by the drugs colchicine and taxol. The jupiter cell lines and fly strain should be of wide interest for biologists interested in in vivo analysis of microtubule dynamics.
机译：在这项研究中，我们描述了一种新的果蝇蛋白木星，它与包括TAU，MAP2，MAP4在内的几种结构性微管相关蛋白（MAPs）具有相同的特性。木星是具有高净正电荷的可溶性未折叠分子，富含甘氨酸。它在序列PPGG周围具有两个简并的重复序列，被富含丝氨酸的区域隔开。木星在体外与微管结合，并与绿色荧光蛋白（GFP）融合，是跟踪体内微管动力学的极佳标记。在通过“蛋白质诱捕”技术生成的木星转基因果蝇菌株中，木星：GFP融合蛋白在不同发育阶段通过细胞周期定位于微管网络。我们在幼胚，幼虫神经系统，眼睛感光细胞前体和成年卵巢中发现了特别高的Jupiter：GFP浓度。此外，从木星：gfp胚胎中，我们建立了两个永久细胞系，在整个细胞周期中均呈现强荧光微管。在这些细胞中，Jupiter：GFP融合蛋白的分布在通过秋水仙碱和紫杉醇进行治疗后可再现微管行为。对于对体内微管动力学分析感兴趣的生物学家，木星细胞系和果蝇应引起广泛关注。

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