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首页> 外文期刊>Cell motility and the cytoskeleton >A role for cortactin in Listeria monocytogenes invasion of NIH 3T3 cells, but not in its intracellular motility.
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A role for cortactin in Listeria monocytogenes invasion of NIH 3T3 cells, but not in its intracellular motility.

机译：cortactin在NIH 3T3细胞单核细胞增多性李斯特菌入侵中的作用，但在其细胞内运动中没有作用。

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Cortactin is an F-actin binding protein that binds to the Arp2/3 complex, stimulates its actin nucleation activity, and inhibits actin filament debranching. Using RNA interference directed against cortactin, we explored the importance of cortactin for several processes involving dynamic actin assembly. Silencing cortactin expression was efficiently achieved in HeLa and NIH 3T3 cells, with less than 5% of cortactin expression in siRNA-treated cells. Surprisingly, endocytosis in HeLa and NIH 3T3 cells, and cell migration rates, were not altered by RNAi-mediated cortactin silencing. Listeria utilizes actin-based motility to move within and spread among mammalian host cells; its actin-clouds and tails recruit cortactin. We explored the role of cortactin during the Listeria life cycle in cortactin "knockdown" NIH 3T3 cells. Interestingly, cortactin siRNA-treated cells showed a significant reduction in the efficiency of the bacteria invasion in NIH 3T3 cells. However, cortactin depletion did not interfere with assembly of Listeria actin clouds or actin tails, or Listeria intracellular motility or speed. Therefore, our findings suggest that cortactin plays a role in Listeria internalization, but not in the formation of actin clouds and tails, or in bacteria intracellular motility.
机译：Cortactin是一种F-肌动蛋白结合蛋白，可与Arp2 / 3复合物结合，刺激其肌动蛋白成核活性，并抑制肌动蛋白丝的脱支。使用针对cortactin的RNA干扰，我们探​​讨了cortactin在涉及动态肌动蛋白组装的几个过程中的重要性。在HeLa和NIH 3T3细胞中可有效实现沉默cortactin的表达，而在siRNA处理的细胞中cortactin的表达少于5％。令人惊讶的是，RNAi介导的cortactin沉默并未改变HeLa和NIH 3T3细胞的内吞作用以及细胞迁移速率。李斯特菌利用基于肌动蛋白的动力在哺乳动物宿主细胞内移动并在其中传播。它的肌动蛋白云团和尾巴吸收了cortactin。我们探讨了在李斯特菌生命周期中的皮质激素在皮质激素“击倒” NIH 3T3细胞中的作用。有趣的是，用cortactin siRNA处理的细胞在NIH 3T3细胞中细菌入侵的效率显着降低。但是，Cortactin的消耗不会干扰李斯特菌肌动蛋白云或肌动蛋白尾巴的组装或李斯特菌细胞内的运动性或速度。因此，我们的发现表明，cortactin在李斯特菌内在化中起作用，但在肌动蛋白云和尾巴的形成或细菌细胞内运动中不起作用。

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