• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell motility and the cytoskeleton >Regulation of microtubule dynamics in 3T3 fibroblasts by Rho family GTPases.
【24h】

Regulation of microtubule dynamics in 3T3 fibroblasts by Rho family GTPases.

机译：Rho家族GTPases对3T3成纤维细胞中微管动力学的调节。

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To get insight into the action of Rho GTPases on the microtubule system we investigated the effects of Cdc42, Rac1, and RhoA on the dynamics of microtubules in Swiss 3T3 fibroblasts. In control cells microtubule ends were dynamic: plus ends frequently switched between growth, shortening and pauses; the growth phase predominated over shortening. Free minus ends of microtubules depolymerized rapidly and never grew. Free microtubules were short-lived, and the microtubule network was organized into a radial array. In serum-starved cells microtubule ends became more stable: although plus ends still transited between growth and shortening, polymerization and depolymerization excursions became shorter and balanced each other. Microtubule minus ends were also stabilized. Consequently lifespan of free microtubules increased and microtubule array changed its radial pattern into a random one. Activation of Cdc42 and Rac1 in serum-starved cells promoted dynamic behavior of microtubule plus and minus ends, while inhibition of these GTPases in serum-grown cells suppressed microtubule dynamics and mimicked all effects of serum starvation. Activation of RhoA in serum-grown cells had effects similar to Cdc42 /Rac1 inactivation: it suppressed the dynamics of plus and minus ends, reduced the length of growth and shrinking episodes, and disrupted the radial organization of microtubules. However, in contrast to Cdc42 and Rac1 inactivation, active RhoA had no effect on the balance between microtubule growth and shortening. We conclude that Cdc42 and Rac1 have similar stimulating effects on microtubule dynamics while RhoA acts in an opposite way.
机译：为了深入了解Rho GTPases对微管系统的作用，我们研究了Cdc42，Rac1和RhoA对瑞士3T3成纤维细胞微管动力学的影响。在对照细胞中，微管末端是动态的：其末端经常在生长，缩短和停顿之间切换;生长阶段主要是缩短。微管的自由负端迅速解聚，并且从未生长。游离的微管寿命短，并且微管网络组织成放射状阵列。在缺乏血清的细胞中，微管末端变得更稳定：尽管其末端仍在生长和缩短之间过渡，但是聚合和解聚的偏移变得更短且彼此平衡。微管负端也稳定。结果，游离微管的寿命增加并且微管阵列将其放射状改变为随机的放射状。血清饥饿的细胞中Cdc42和Rac1的激活促进了微管正负末端的动态行为，而抑制血清生长的细胞中的这些GTPases抑制了微管的动力学并模仿了血清饥饿的所有作用。血清生长细胞中RhoA的激活具有类似于Cdc42 / Rac1失活的作用：它抑制了正负末端的动力学，减少了生长和收缩发作的时间，并破坏了微管的放射状组织。但是，与Cdc42和Rac1失活相反，活性RhoA对微管生长和缩短之间的平衡没有影响。我们得出结论，Cdc42和Rac1对微管动力学具有相似的刺激作用，而RhoA则以相反的方式起作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号