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首页> 外文期刊>Cell motility and the cytoskeleton >Adenomatous polyposis coli localization is both cell type and cell context dependent.
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Adenomatous polyposis coli localization is both cell type and cell context dependent.

机译：腺瘤性息肉病大肠杆菌的定位既取决于细胞类型，又取决于细胞环境。

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The adenomatous polyposis coli (APC) tumor suppressor protein is mutated in most colorectal carcinomas. In addition to its role in WNT signaling it is proposed to be involved in both cell migration and mitosis. Although a variety of studies have shown an APC localization along lateral membranes of adjacent epithelial cells the existence of a cortical APC localization in mammalian cells remains controversial. To address this we have used matched rat epithelial (NRK-52E) and fibroblast (NRK-49F) cell lines to investigate the localization of APC. Subconfluent cultures of NRK-52E and -49F cells displayed microtubule-associated APC populations by immunostaining. However, confluent NRK-52E, but not -49F monolayers, exhibited a cortical APC distribution. Cortical APC localized in close proximity to a number of cell junction proteins in a microtubule-independent manner while calcium switch experiments suggested that APC was recruited to the cortex only when junction assembly was complete. Confluent NRK-49F and-52E cells also showed contrasting APC localizations in response to monolayer wounding. Our data suggests APC cortical localization is a feature of confluent epithelioid cells and that the subcellular distribution of APC is therefore dependent upon both cell type and context.
机译：在大多数大肠癌中，腺瘤性息肉病大肠杆菌（APC）抑癌蛋白发生了突变。除了其在WNT信号传导中的作用外，还建议它参与细胞迁移和有丝分裂。尽管各种各样的研究表明，APC沿着相邻上皮细胞的侧膜定位，但哺乳动物细胞中皮质APC定位的存在仍存在争议。为了解决这个问题，我们使用了匹配的大鼠上皮（NRK-52E）和成纤维细胞（NRK-49F）细胞系来研究APC的定位。 NRK-52E和-49F细胞的亚融合培养通过免疫染色显示了微管相关的APC群体。但是，汇合的NRK-52E，而不是-49F单层，表现出皮质APC分布。皮质APC以与微管无关的方式定位在许多细胞连接蛋白的附近，而钙转换实验表明，只有在连接组装完成时，APC才被募集到皮质。融合的NRK-49F和-52E细胞还显示出响应于单层伤口的APC定位相反。我们的数据表明APC皮质定位是融合的上皮样细胞的特征，因此APC的亚细胞分布取决于细胞类型和环境。

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