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首页> 外文期刊>Cell motility and the cytoskeleton >Cell distribution of stress fibres in response to the geometry of the adhesive environment.
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Cell distribution of stress fibres in response to the geometry of the adhesive environment.

机译：应力纤维的细胞分布响应于粘合环境的几何形状。

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摘要

Cells display a large variety of shapes when plated in classical culture conditions despite their belonging to a common cell type. These shapes are transitory, since cells permanently disassemble and reassemble their cytoskeleton while moving. Adhesive micropatterns are commonly used to confine cell shape within a given geometry. In addition the micropattern can be designed so as to impose cells to spread upon adhesive and nonadhesive areas. Modulation of the pattern geometry allows the analysis of the mechanisms governing the determination of cell shape in response to external adhesive conditions. In this study, we show that the acquisition of cell shape follows two stages where initially the cell forms contact with the micropattern. Here, the most distal contacts made by the cell with the micropattern define the apices of the cell shape. Then secondly, the cell borders that link two apices move so as to minimise the distance between the two apices. In these cell borders, the absence of an underlying adhesive substrate is overcome by stress fibres forming between the apices, which in turn are marked by an accumulation of focal adhesions. By inhibiting myosin function, cell borders on nonadhesive zones become more concave, suggesting that the stress fibres work against the membrane tension in the cell border. Moreover, this suggested that traction forces are unevenly distributed in stationary, nonmigrating, cells. By comparing the stress fibres in cells with one, two, or three nonadherent cell borders it was reasoned that stress fibre strength is inversely proportional to number. We conclude that cells of a given area can generate the same total sum of tractional forces but that these tractional forces are differently spaced depending on the spatial distribution of its adherence contacts.
机译：尽管细胞属于一种常见的细胞类型，但在经典培养条件下进行铺板时，它们会显示出多种形状。这些形状是暂时的，因为细胞在移动时会永久分解并重新组装其细胞骨架。粘合微图案通常用于将细胞形状限制在给定的几何形状内。另外，可以将微图案设计成将细胞强加在粘性和非粘性区域上。图案几何形状的调制允许响应于外部粘附条件来分析控制确定细胞形状的机制。在这项研究中，我们表明，细胞形状的获取遵循两个阶段，其中最初细胞形成与微图案的接触。在此，细胞与微图案形成的最远端接触定义了细胞形状的顶点。然后，第二个连接两个顶点的细胞边界移动，以最小化两个顶点之间的距离。在这些细胞边界中，通过在顶点之间形成应力纤维克服了下面的粘合基底的缺乏，而应力纤维又以粘着斑的积累为特征。通过抑制肌球蛋白功能，非粘附区的细胞边界变得更凹，这表明应力纤维可抵抗细胞边界中的膜张力。此外，这表明牵引力在静止的，未迁移的单元中分布不均匀。通过将细胞中的应力纤维与一个，两个或三个非粘附细胞边界进行比较，可以推断出应力纤维的强度与数量成反比。我们得出的结论是，给定区域的单元格可以生成相同的总牵引力，但这些牵引力的间隔取决于其附着接触的空间分布。

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