RNA-Binding Properties of the Plant Protein Nt-4/1

S. S. Makarova; A. G. Solovyev; S. Y. Morozov

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RNA-Binding Properties of the Plant Protein Nt-4/1

机译：植物蛋白Nt-4 / 1的RNA结合特性。

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The tobacco a-helical protein Nt-4/1 with unknown function forms ribonucleoprotein (RNP) complexes in vitro. Results obtained by retardation of RNP complexes in agarose gel were confirmed by Western—Northern hybridization. Several deletion and point mutants of Nt-4/1 were constructed, and the RNA-binding site was mapped in a positively charged region of the C-terminal domain of the protein. The results of this study and those described earlier support our hypothesis of the participation of Nt-4/1 protein in spreading RNA-containing pathogens in the plant.

机译：功能未知的烟草α-螺旋蛋白Nt-4 / 1在体外形成核糖核蛋白（RNP）复合物。 Western-Northern杂交证实了琼脂糖凝胶中RNP复合物阻滞所获得的结果。构建了几个Nt-4 / 1的缺失和点突变体，并将RNA结合位点定位在蛋白质C端结构域的带正电荷的区域。这项研究的结果以及先前所述的结果支持了我们的假设，即Nt-4 / 1蛋白参与了植物中含RNA传播的病原体的传播。

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《Biochemistry》 |2014年第7期|共10页
作者
S. S. Makarova; A. G. Solovyev; S. Y. Morozov;
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正文语种 eng
中图分类生物化学;
关键词
protein Nt-4/1; potato spindle tuber viroid; RNA binding;

机译：Nt-4 / 1蛋白;马铃薯纺锤块茎类病毒;RNA结合;

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1. RNA-Binding Properties of the Plant Protein Nt-4/1 [J] . S. S. Makarova, A. G. Solovyev, S. Y. Morozov Biochemistry . 2014,第7期

机译：植物蛋白Nt-4 / 1的RNA结合特性。
2. Mechanistic Insight into Pentatricopeptide Repeat Proteins as Sequence-Specific RNA-Binding Proteins for Organellar RNAs in Plants [J] . Takahiro Nakamura123* Yusuke Yagi12 and Keiko Kobayashi12 Plant and Cell Physiology . 2012,第7期

机译：机械洞察五肽重复蛋白作为植物器官RNA的序列特异性RNA结合蛋白。
3. Mechanistic insight into pentatricopeptide repeat proteins as sequence-specific RNA-binding proteins for organellar RNAs in plants. [J] . Nakamura T., Yagi Y., Kobayashi K. Plant and cell physiology . 2012,第7期

机译：对五肽肽重复蛋白作为植物细胞内RNA的序列特异性RNA结合蛋白的机理研究。
4. Preliminary Study on the RNA-Binding Properties of BYDV-GAV and BYDV-GPV Movement Proteins [C] . Z. Xia, Y. Zhang, K. Liu, International Symposium of Barley Yellow Dwarf Disease . 2001

机译：BYDV-GAV和BYDV-GPV运动蛋白RNA结合特性的初步研究
5. Characterization of two RNA-binding proteins containing RNA-Recognition Motif (RRM)-type RNA-binding domains from mouse brain. [D] . Inman, Michael Verne. 1999

机译：表征两个RNA结合蛋白的小鼠大脑中的RNA识别基序（RRM）型RNA结合域。
6. Evolution of the Small Family of Alternative Splicing Modulators Nuclear Speckle RNA-Binding Proteins in Plants [O] . Leandro Lucero, Jeremie Bazin, Johan Rodriguez Melo, 2020

机译：植物的选择性剪接调节剂小家族的演变核斑点RNA结合蛋白在植物中。
7. Molecular evolution of RRM-containing proteins and glycine-rich RNA-binding proteins in plants [O] . Judith L. Gomez-Porras, Martin Lewinski, Diego Mauricio Ria#xf1, 2011

机译：植物中含有RRM的蛋白质和富含甘氨酸的RNA结合蛋白的分子进化
8. RNA-Binding Proteins as Novel Oncoproteins and Tumor Suppressors in Breast Cancer [R] . Brewer, G. 2006

机译：RNa结合蛋白作为乳腺癌中的新型癌蛋白和肿瘤抑制因子

RNA-Binding Properties of the Plant Protein Nt-4/1

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