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首页> 外文期刊>Cell and Tissue Research >Coxsackievirus and adenovirus receptor-positive cells compose the putative stem/progenitor cell niches in the marginal cell layer and parenchyma of the rat anterior pituitary
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Coxsackievirus and adenovirus receptor-positive cells compose the putative stem/progenitor cell niches in the marginal cell layer and parenchyma of the rat anterior pituitary

机译：柯萨奇病毒和腺病毒受体阳性细胞组成了大鼠垂体前叶的边缘细胞层和实质中的推定干/祖细胞壁ni

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摘要

The pituitary gland is a slow generative tissue but actively responds to demands by changing homeostasis. The marginal cell layer (MCL) facing the residual lumen has long been indicated as a stem/progenitor cell niche of the pituitary. On the other hand, the coxsackievirus and adenovirus receptor (CAR), which localizes at the tight-junction of the polarized epithelium, is known to participate in the development, differentiation and regeneration of specified tissues. The present study attempts to characterize the cells lining the MCL during pituitary development by immunohistochemistry of CAR. Consequently, we found that CAR localizes in an apical surface of the single cell layer facing the oral cavity in the invaginating oral epithelium on rat embryonic day (E) 11.5. On E13.5, when this single layer constructs the MCL in the pituitary primordium Rathke's pouch, CAR-positive cells occupied the MCL and this localization pattern of CAR was persistently maintained throughout life. Moreover, clusters of CAR-positive cells were also found in the parenchyma. CAR-positive cells were positive for stem/progenitor cell markers sex-determining region Y-box 2 (SOX2) and epithelial calcium-dependent adhesion (E-cadherin). However, prior to the postnatal growth wave, cells positive for CAR in the basolateral surface constructed multiple cell layers beneath the MCL and cell-type transition to a putative migratory cell phenotype by fading of SOX2 and E-cadherin occurred, suggesting the composition of new putative niches in the parenchyma. These data, together with our previous reports, suggest that CAR-positive cells are pituitary stem/progenitor cells and compose putative stem/progenitor cell niches in the MCL and parenchyma.
机译：垂体是一个缓慢的生殖组织，但通过改变稳态来积极响应需求。长期以来，面向残余腔的边缘细胞层（MCL）一直被认为是垂体的干/祖细胞位。另一方面，已知柯萨奇病毒和腺病毒受体（CAR）位于极化上皮的紧密连接处，参与特定组织的发育，分化和再生。本研究试图通过CAR的免疫组织化学来表征垂体发育过程中位于MCL衬里的细胞。因此，我们发现CAR定位于大鼠胚胎日（E）11.5时，在侵袭性口腔上皮中面向口腔的单个细胞层的顶表面中。在E13.5上，当此单层在垂体原基Rathke囊中构建MCL时，CAR阳性细胞占据了MCL，并且CAR的这种定位模式在整个生命中都得到了持续维持。此外，在薄壁组织中还发现了CAR阳性细胞簇。 CAR阳性细胞的干/祖细胞标记性别决定区域Y-box 2（SOX2）和上皮钙依赖性粘附（E-cadherin）呈阳性。然而，在产后生长波之前，基底外侧表面CAR阳性的细胞在MCL下方构建了多个细胞层，并且由于SOX2和E-钙粘着蛋白的褪色而发生了细胞类型向假定的迁移细胞表型的转变，这提示了新的组成。薄壁组织中的假定壁ni。这些数据以及我们以前的报道表明，CAR阳性细胞是垂体干/祖细胞，在MCL和薄壁组织中构成假定的干/祖细胞cell。

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