...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell and Tissue Research >Expression of neuropeptides and anoctamin 1 in the embryonic and adult zebrafish intestine, revealing neuronal subpopulations and ICC-like cells
【24h】

Expression of neuropeptides and anoctamin 1 in the embryonic and adult zebrafish intestine, revealing neuronal subpopulations and ICC-like cells

机译：胚胎和成年斑马鱼肠道中神经肽和八环素1的表达，揭示了神经元亚群和ICC样细胞

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This immunohistochemical study in zebrafish aims to extend the neurochemical characterization of enteric neuronal subpopulations and to validate a marker for identification of interstitial cells of Cajal (ICC). The expression of neuropeptides and anoctamin 1 (Ano1), a selective ICC marker in mammals, was analyzed in both embryonic and adult intestine. Neuropeptides were present from 3 days postfertilization (dpf). At 3 dpf, galanin-positive nerve fibers were found in the proximal intestine, while calcitonin gene-related peptide (CGRP)- and substance P-expressing fibers appeared in the distal intestine. At 5 dpf, immunoreactive fibers were present along the entire intestinal length, indicating a well-developed peptidergic innervation at the onset of feeding. In the adult intestine, vasoactive intestinal peptide (VIP), pituitary adenylate cyclase-activating peptide (PACAP), galanin, CGRP and substance P were detected in nerve fibers. Colchicine pretreatment enhanced only VIP and PACAP immunoreactivity. VIP and PACAP were coexpressed in enteric neurons. Colocalization stainings revealed three neuronal subpopulations expressing VIP and PACAP: a nitrergic noncholinergic subpopulation, a serotonergic subpopulation and a subpopulation expressing no other markers. Ano1-immunostaining revealed a 3-dimensional network in the adult intestine containing multipolar cells at the myenteric plexus and bipolar cells interspersed between circular smooth muscle cells. Ano1 immunoreactivity first appeared at 3 dpf, indicative of the onset of proliferation of ICC-like cells. It is shown that the Ano1 antiserum is a selective marker of ICC-like cells in the zebrafish intestine. Finally, it is hypothesized that ICC-like cells mediate the spontaneous regular activity of the embryonic intestine.
机译：这项在斑马鱼中的免疫组织化学研究旨在扩展肠神经元亚群的神经化学特性，并验证用于鉴定Cajal间质细胞（ICC）的标记。分析了胚胎和成年肠道中神经肽和选择性ICC标记物Octamin 1（Ano1）的表达。受精后3天（dpf）出现神经肽。在3 dpf时，在近端肠中发现了甘丙肽阳性神经纤维，而在远端肠中出现了降钙素基因相关肽（CGRP）和P物质表达纤维。在5 dpf时，整个肠段均存在免疫反应性纤维，表明在进食时已充分发育了肽能神经。在成人肠中，在神经纤维中检测到血管活性肠肽（VIP），垂体腺苷酸环化酶激活肽（PACAP），甘丙肽，CGRP和P物质。秋水仙碱预处理仅增强VIP和PACAP免疫反应性。 VIP和PACAP在肠神经元中共表达。共定位染色揭示了三个表达VIP和PACAP的神经元亚群：硝化非胆碱能亚群，血清素能亚群和不表达其他标记的亚群。 Ano1免疫染色揭示了在成年肠道中的3维网络，其中包含位于肌间神经丛的多极细胞和散布在圆形平滑肌细胞之间的双极细胞。 Ano1免疫反应性首先出现在3 dpf，这表明ICC样细胞开始增殖。结果表明，Ano1抗血清是斑马鱼肠中ICC样细胞的选择性标记。最后，假设ICC样细胞介导了胚胎小肠的自发规则活性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号